Deep profiling of cellular heterogeneity by emerging singlecell proteomic technologies

L Yang, J George, J Wang - Proteomics, 2020 - Wiley Online Library
… and cellcell interactions and highlighting tumor heterogeneity. However, the mass cytometry
approachesprobes. Another distinct advantage of the droplet-based microfluidics for …

A pan-cancer blueprint of the heterogeneous tumor microenvironment revealed by single-cell profiling

J Qian, S Olbrecht, B Boeckx, H Vos, D Laoui… - Cell research, 2020 - nature.com
… we profile 233,591 single cells from patients with lung, colorectal, ovary and breast cancer (n
= 36) and construct a pan-cancer … Our approach was less successful for cancer cells, which …

Single-cell analysis of circulating tumor cells: why heterogeneity matters

SB Lim, CT Lim, WT Lim - Cancers, 2019 - mdpi.com
… tool for assessing tumor heterogeneity and for revealing … to in-depth sequencing to probe
only CTCs with yields of DNA … resolving cellular heterogeneity, single-cell approaches may …

An integrated gene expression landscape profiling approach to identify lung tumor endothelial cell heterogeneity and angiogenic candidates

J Goveia, K Rohlenova, F Taverna, L Treps… - Cancer cell, 2020 - cell.com
… of endothelial cells (ECs) to block tumorcancer using single-cell RNA sequencing in
combination with orthogonal bulk multi-omics approaches to provide a molecular atlas of tumor EC (…

Applications of single-cell sequencing in cancer research: progress and perspectives

Y Lei, R Tang, J Xu, W Wang, B Zhang, J Liu… - Journal of hematology & …, 2021 - Springer
cells and immune cells, tumor heterogeneity, circulating … and immune cells because it can
probe cellular and … The 10X Genomics platform, which is based on a microfluidics approach, is …

Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis

K Xu, R Wang, H Xie, L Hu, C Wang, J Xu, C Zhu, Y Liu… - Oncogenesis, 2021 - nature.com
… future studies on tumor heterogeneity and pave … probe the genomic profile of breast cancer,
we applied inferCNV algorithm to analyze the copy-number variants (CNVs) of a single cell

Tumor heterogeneity in glioblastomas: from light microscopy to molecular pathology

AP Becker, BE Sells, SJ Haque, A Chakravarti - Cancers, 2021 - mdpi.com
… molecular alterations in DNA, RNA, and proteins observed at individual cells. … -tumor
heterogeneity of GBMs, which directly affects prognosis and response to treatment. Our approach

Inter-and intra-tumor heterogeneity of metastatic prostate cancer determined by digital spatial gene expression profiling

L Brady, M Kriner, I Coleman, C Morrissey… - Nature …, 2021 - nature.com
probes (median of 10 probes/target mRNA) for each gene of interest for a total of 18,120
probes, … In this study, we used a robust approach for quantitative spatial molecular profiling to …

Addressing cellular heterogeneity in tumor and circulation for refined prognostication

SB Lim, T Yeo, WD Lee, AAS Bhagat… - Proceedings of the …, 2019 - National Acad Sciences
… an unanticipated impact of intratumor heterogeneity (ITH) … probe heterogeneity within the
CTC population, we further isolated and analyzed 24 single cells of 7 different lung cancer cell

Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states

H Song, HNW Weinstein, P Allegakoen… - Nature …, 2022 - nature.com
… To probe the diversity of cell types and transcriptional states … the cellular state heterogeneity
revealed by single-cell … prostate cancer-associated club cells, we used established methods