Towards a Comprehensive Optical Connectome at Single Synapse Resolution via Expansion Microscopy
MA Sneve, KD Piatkevich - Frontiers in Synaptic Neuroscience, 2022 - frontiersin.org
… Many tools are variations of this general technique and are widely used both for live imaging
and … For example, the worm connectome has been combined with ablation experiments to …
and … For example, the worm connectome has been combined with ablation experiments to …
Microfluidic devices for imaging and manipulation of C. elegans
AS Clark, J Huayta, KS Arulalan… - Micro and Nano Systems …, 2021 - Elsevier
… Recently, new platforms have integrated imaging with long-term culture, starting from larval
stages to adulthood, and the complete automation of chemical or mechanosensory stimuli [20…
stages to adulthood, and the complete automation of chemical or mechanosensory stimuli [20…
A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
J Dubey, S Mondal, SP Koushika - JoVE (Journal of Visualized …, 2022 - jove.com
… elegans in presence of a continuous food supply for up to 36 h. The growth and imaging
device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular …
device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular …
In vivo imaging of synaptogenesis
… the benefits of using appropriate animal models for live imaging. … model organisms like
Caenorhabditis elegans and Drosophila … Both methods can be used for long-term imaging over …
Caenorhabditis elegans and Drosophila … Both methods can be used for long-term imaging over …
The connectome of the Caenorhabditis elegans pharynx
… Consistent with the strongest ablation phenotype in the pharynx … Only a single synapse,
NSM→I5, did not have matching … Einstein College of Medicine for long-term curation and study. …
NSM→I5, did not have matching … Einstein College of Medicine for long-term curation and study. …
Caenorhabditis elegans-on-a-chip: microfluidic platforms for high-resolution imaging and phenotyping
S Mondal, A Ben-Yakar - Organ-on-a-chip, 2020 - Elsevier
… These devices have contributed to our knowledge in areas such as long-term disease
progression, in vivo nerve regeneration, and aging research in live animals. The assays typically …
progression, in vivo nerve regeneration, and aging research in live animals. The assays typically …
[HTML][HTML] Automation of C. elegans lifespan measurement
DP Felker, CE Robbins, MA McCormick - Translational medicine of aging, 2020 - Elsevier
… The nematode Caenorhabditis elegans [7] has been proven to be an extremely useful model
in … Agar plate is flipped upside-down and placed on scanner to allow for image capture for …
in … Agar plate is flipped upside-down and placed on scanner to allow for image capture for …
[PDF][PDF] Regulation of neurotransmitter release and synaptic plasticity at the single synapse level
HS Langley - 2022 - discovery.ucl.ac.uk
… imaging methods often do not have sufficient temporal precision to resolve fast synaptic
release events. In addition, existing methods which can resolve single synapse … imaging and …
release events. In addition, existing methods which can resolve single synapse … imaging and …
Microfluidic Technologies for High Throughput Screening Through Sorting and On-Chip Culture of C. elegans
D Midkiff, A San-Miguel - Molecules, 2019 - mdpi.com
… elegans is suitable for long-term studies of lifespan and aging … to operate at the resolution
of a single synapse [89]. This … laser ablation as well as the subsequent neuronal imaging or …
of a single synapse [89]. This … laser ablation as well as the subsequent neuronal imaging or …
Molecular mechanisms governing axonal transport: a C. elegans perspective
A Vasudevan, SP Koushika - Nature's Gift to Neuroscience, 2022 - taylorfrancis.com
… One of its strengths is its transparency, that allows for the application of live imaging
techniques in intact animals. In this review, we discuss the principles of axonal transport and …
techniques in intact animals. In this review, we discuss the principles of axonal transport and …