[HTML][HTML] A multiplex PCR assay for detection of Vibrio vulnificus, Aeromonas hydrophila, methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, and …

YH Tsai, PH Chen, PA Yu, CL Chen, LT Kuo… - International Journal of …, 2019 - Elsevier
… a multiplex PCR method for the simultaneous detection of five … NF by using a novel combination
of species-specific genes. … Application of the rpoS gene for species-specific detection of …

[HTML][HTML] Rapid identification of pathogens involved in pediatric osteoarticular infections by multiplex PCR

C Gan, J Hu, Q Cao, R Zhao, Y Li, Z Wang… - Annals of …, 2020 - ncbi.nlm.nih.gov
… and mecA for methicillin-resistant Staphylococcus aureus, … The multiplex PCR was first
evaluated with 39 isolated clinical … multiplex PCR assay was designed to simultaneously detect

An array of multiplex PCR assays for detection of staphylococcal chromosomal cassette mec (SCCmec) types among staphylococcal isolates

D Bhowmik, BJ Das, P Pandey, S Chetri… - Journal of …, 2019 - Elsevier
detection of SCCmec elements. So, here we have described the use of three sets of multiplex
PCR … in the genome of methicillin resistant Staphylococcus aureus is designated as the …

Triplex PCR combined with magnetic separation strategy for rapid and specific detection of methicillin-resistant Staphylococcus aureus in hospital samples

Z Wang, G Xie, G Chen, X Gao, J Li, Z Xie, H Xu - Microchemical Journal, 2021 - Elsevier
detection of pathogens is polymerase chain reaction (PCR)[18]. The multiplex PCR (mPCR)
developed in this study can detect … culture method, the detection efficiency was improved. …

[HTML][HTML] Molecular detection and typing of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci isolated from cattle …

N Venugopal, S Mitra, R Tewari, F Ganaie… - Veterinary …, 2019 - ncbi.nlm.nih.gov
… by PCR identification of mecA and mecC resistance determinants. Species-specific
identification of mecA positive isolates was conducted by multiplex PCR. The unidentified …

… -Based biosensor coupled with multiplex loop-mediated isothermal amplification for detection of Staphylococcus aureus and identification of methicillin-resistant S …

L Jiang, X Li, R Gu, D Mu - Infection and Drug Resistance, 2020 - Taylor & Francis
Methicillin-resistant Staphylococcus aureus(MRSA) infection has become a serious worldwide
problem, with high mortality and morbidity since the 1970s. Citation3 As it is reported, in …

[HTML][HTML] Real-time PCR assay for detection of Staphylococcus aureus, Panton-Valentine Leucocidin and Methicillin Resistance directly from clinical samples

L Galia, M Ligozzi, A Bertoncelli, A Mazzariol - AIMS microbiology, 2019 - ncbi.nlm.nih.gov
… Rapid detection of Methicillin Resistant Staphylococcus aureus (MRSA) is an … PCR
assay to detect directly nuc, pvl, and mecA genes. The assay is able to perform identification

[HTML][HTML] … values of multiplex loop-mediated isothermal amplification and multiplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus …

S Kashani, AH Alvandi, R Abiri - Jundishapur Journal of Microbiology, 2020 - brieflands.com
… develop detection assays for Staphylococcal mecA and spa using multiplex PCR and LAMP.
… aureus were confirmed to the species level using biochemical tests and multiplex PCR and …

The rapid and visual detection of methicillin-susceptible and methicillin-resistant Staphylococcus aureus using multiplex loop-mediated isothermal amplification …

X Chen, K Ma, X Yi, L Xiong, Y Wang, S Li - Antimicrobial Resistance & …, 2020 - Springer
… In recent decades, many molecular methods, such as polymerase chain reaction (PCR),
multiplex PCR and real-time PCR, have been applied for detecting MSSA and MRSA isolates […

[HTML][HTML] Comparison of PCR and phenotypic methods for the detection of methicillin resistant Staphylococcus aureus

A Madhavan, A Sachu, A Balakrishnan… - Iranian Journal of …, 2021 - ncbi.nlm.nih.gov
… Resistance to methicillin in methicillin resistant strains of Staphylococcus aureus (MRSA)
is due to the presence of mec-A gene, which encodes a low affinity penicillin binding protein (…