Advances in stable isotope labeling: dynamic labeling for spatial and temporal proteomic analysis

NC Beller, AB Hummon - Molecular omics, 2022 - pubs.rsc.org
… The use of isotopically labeled amino acids was developed … Stable Isotope Labeling by
Amino Acids in Cell culture (SILAC… forms of heavy isotopic labels to quantify relative differences

… -and autophagy-associated molecules in human lung cancer A549 cells in response to cisplatin treatment using stable isotope labeling with amino acids in cell culture

Z Wang, G Liu, J Jiang - International Journal of …, 2019 - spandidos-publications.com
Stable isotope labeling by amino acids in cell culture (SILAC) is effective in distinguishing
… The incorporation of stable isotopes facilitates the quantitative recognition of the differences

Advances and applications of stable isotope labeling-based methods for proteome relative quantitation

J Liu, Y Shan, Y Zhou, Z Liang, L Zhang… - TrAC Trends in Analytical …, 2020 - Elsevier
… with different stable isotope reagents are combined into the same LC-MS/MS run [15]. … use
in proteome-wide quantitation. Stable isotope labeling by amino acids in cell culture (SILAC) […

Spatial stable isotopic labeling by amino acids in cell culture: Pulse-chase labeling of three-dimensional multicellular spheroids for global proteome analysis

NC Beller, JK Lukowski, KR Ludwig… - Analytical …, 2021 - ACS Publications
isotopic labels could be incorporated into the proteins of the individual spheroid cellular
layers by using timed cell culture … for the comparison of different isotopic labels among each of …

… head and neck squamous cell carcinoma? New insights from stable isotope labeling with amino acids in cell culture-based quantitative tyrosine phosphoproteomics

AP Jain, A Radhakrishnan, S Pinto, K Patel… - OMICS: A Journal of …, 2021 - liebertpub.com
… erlotinib-sensitive) cells using stable isotope labeling with amino acids in cell culture (SILAC)…
We used a quantitative tyrosine phosphoproteomics approach to compare SCC-R (erlotinib-…

Characterization of the primary human trophoblast cell secretome using stable isotope labeling with amino acids in cell culture

FJ Rosario, S Pardo, TM Michelsen… - Frontiers in Cell and …, 2021 - frontiersin.org
… We also examined the relative synthesis rates of different subunits … different subunits in
the PHT secretome are coordinated. However, ribosomal subunits were synthesized at different

Mass-Spectrometric Evaluation of the African Swine Fever Virus-Induced Host Shutoff Using Dynamic Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC …

E Wöhnke, BG Klupp, S Blome, TC Mettenleiter… - Viruses, 2023 - mdpi.com
… of relative protein synthesis rates using a mass spectrometric approach based on stable
isotope labeling with amino acids in cell culture (SILAC). The impact of ASFV infection on the …

Stable isotope labeling of amino acids in flies (SILAF) reveals differential phosphorylation of mitochondrial proteins upon loss of OXPHOS subunits

FA Rosenberger, I Atanassov, D Moore… - … & Cellular Proteomics, 2021 - ASBMB
… in flies), TCA (tricarboxylic acid), TiO2 (titanium dioxide… Stable-isotope labeling of amino
acids in cell culture, known as SILAC, uses nonradioactive isotopic labeling to detect differences

HyperSCP: Combining isotopic and isobaric labeling for higher throughput single-cell proteomics

Y Liang, T Truong, AJ Saxton, H Boekweg… - Analytical …, 2023 - ACS Publications
… of labeling to achieve multiplicative scaling for higher throughput. Two-plex stable isotope
labeling of amino acids in cell culture (… , isotopic labels create a mass difference between …

Using affinity purification coupled with stable isotope labeling by amino acids in cell culture quantitative mass spectrometry to identify novel interactors/substrates of …

A Morettin, J Bourassa, K Mahadevan… - Methods, 2020 - Elsevier
… Here, we describe the use of affinity purification (AP) coupled with stable isotope labeling
with amino acids in cell culture (SILAC) quantitative mass spectrometry (MS) to identify protein …