… RN-01 levansucrase (oligosaccharide producing levansucrase, OPL) can produce LFOs as
… Structural analysis of OPL and the K82H/N83R mutant. (A) Circular dichroism spectra of the …

… subtilis levansucrase) is partially buried and therefore not … By diminishing the levansucrase
ability to produce larger products, … and purification of these sugars was not optimized in this …

… and structural analysis to enhance the LFOS production. High… ) analysis indicated that Y237S
variant efficiently produced a … and sucrose in the reactions was optimized. Finally, prebiotic …

… A step by step optimization process concerning the effect of … Production of
levansucrase from Bacillus subtilis NRC 33a and enzymic synthesis of levan and fructo-oligosaccharides …

… This study developed an effective approach in redesigning the active site of levansucrase
for the production of LFOs with DP up to five by blocking the oligosaccharide binding track with …

… A thermostable levansucrase was able to produce levan and … pullulans cells for fructo-oligosaccharides
production. Biochem … Structural analyses and immunomodulatory properties of …

In the present study, the cross-linked enzyme aggregates (CLEAs) of Y246S variant levansucrase
from Bacillus licheniformis RN-01, known as oligosaccharide-producing levansucrase …

… licheniformis RN-01 levansucrase (oligosaccharide-producing levansucrase, OPL) was …
To enhance OPL structural stability, this study employed molecular dynamics (AMBER) to …

… This study was conducted to optimize oligosaccharide production using response surface
methodology, analyze the structure of purified oligosaccharides, and investigate the prebiotic …

… on FOSs, levan and levansucrase production was verified in … (0·2 and 1 vvm), levan production
was similar, at 192·41 and 189·… the levansucrase production, and higher production was …