[HTML][HTML] Structural and molecular kinetic features of activities of DNA polymerases

AA Kuznetsova, OS Fedorova… - International Journal of …, 2022 - mdpi.com
… of action of DNA polymerases. The structural features of different DNA polymerase families
are … conformational dynamics of DNA polymerases from all known polymerase families during …

Elucidating functions of DP1 and DP2 subunits from the Thermococcus kodakarensis family D DNA polymerase

N Takashima, S Ishino, K Oki, M Takafuji, T Yamagami… - Extremophiles, 2019 - Springer
… We reported that neither DP1 nor DP2 showed any DNA polymerase or exonuclease
activity when we discovered PolD in P. furiosus (Uemori et al. 1997). DP2 from P. …

[PDF][PDF] Dynamic exchange of two essential DNA polymerases during replication and after fork arrest

Y Li, Z Chen, LA Matthews, LA Simmons, JS Biteen - Biophysical journal, 2019 - cell.com
… Here, we find that DNA replication arrest by HPUra does not arrest all replisome protein
dynamics. After HPUra treatment, we imaged PolC-PAmCherry, reconstructed PALM super-…

A recurrent cancer-associated substitution in DNA polymerase ε produces a hyperactive enzyme

…, DP Kane, CR Bulock, EA Moore, S Sharma… - Nature …, 2019 - nature.com
… Polε-P301R is a hyperactive DNA polymerase In a search for additional effects of the P301R
substitution, we compared the DNA polymerase activity of wild-type Polε, exo − Polε and …

DNA polymerase δ proofreads errors made by DNA polymerase ε

CR Bulock, X Xing… - Proceedings of the …, 2020 - National Acad Sciences
… selectivity conferred by the polymerase domain and the proofreading activity located in a
separate exonuclease domain. The exonuclease activity of both polymerases can be abolished …

Interlocking activities of DNA polymerase β in the base excision repair pathway

A Kumar, AJ Reed, WJ Zahurancik… - Proceedings of the …, 2022 - National Acad Sciences
DNA polymerase β (Polβ) is presumed to first catalyze gap-filling DNA synthesis through its
DNA polymerase activity … its dRP lyase activity, leaving a nicked DNA substrate for ligation by …

Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis

…, R Jain, RE Johnson, L Prakash, S Prakash… - Nature structural & …, 2020 - nature.com
… This ring-like architecture is unique among multisubunit DNA polymerases and resembles
an uninterrupted daisy chain of protein–protein interactions, wherein Pol32 is the only subunit …

Bioorthogonal information storage in l-DNA with a high-fidelity mirror-image Pfu DNA polymerase

C Fan, Q Deng, TF Zhu - Nature Biotechnology, 2021 - nature.com
… ) DNA polymerase, which possesses the highest fidelity among known natural thermostable
DNA polymerases 23 and is … mass of Pfu DNA polymerase, at 90 kDa, is about twice that of …

Structural dynamics and determinants of 2-aminoadenine specificity in DNA polymerase DpoZ of vibriophage ϕVC8

D Czernecki, H Hu, F Romoli… - Nucleic Acids Research, 2021 - academic.oup.com
… their replication (12), all modern organisms and viruses have evolved specialized proteins
RNA and DNA polymerases (Pol) to efficiently duplicate their genomes. DNA polymerases

Introducing a new bond-forming activity in an archaeal DNA polymerase by structure-guided enzyme redesign

T Aggarwal, WA Hansen, J Hong, A Ganguly… - ACS chemical …, 2022 - ACS Publications
… affect DNA polymerase activity. Both 9NN and 9N-NRQS displayed significantly reduced activity
for extending the canonical 3′-hydroxyl DNA … cause of the reduced DNA extension rate. …