[HTML][HTML] Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute

MS Hossain, R Ahmed, MS Haque, MM Alam… - BMC molecular …, 2019 - Springer
… Compared to all textile fibers except cotton, jute is one of the cheapest and most important
… of reference genes to identify the most suitable internal control for normalization of qRT-PCR

Comparative Expression Studies of Fiber Related Genes in Cotton Spp.

M Nadeem, N Iqbal, U Waheed… - Journal of …, 2021 - corescholar.libraries.wright.edu
… data normalization, 18s rRNA was used as an internal control… A flower with the good and
healthy condition was selected … The qRT-PCR assay was optimized by running a series of …

Transcriptome analysis reveals potential of down-regulated genes in cotton fiber improvement

J Song, B Jia, P Feng, H Xi, W Zhao, H Xi… - Industrial Crops and …, 2024 - Elsevier
… All expression level was normalized by samples. (d) The … , we conducted qRT-PCR analyses
of two selected genes at … -regulated gene pannel suitable for fiber improvement by CRISPR. …

[PDF][PDF] Quantitative real-time PCR based evaluation and validation of reference genes in Gossypium arboreum

KP Raghavendra, R Kumar, J Das… - The Indian Journal of …, 2020 - epubs.icar.org.in
… Through this study, six potential candidate internal control genes from Gossypium arboreum
Suitable internal control genes for qRTPCR normalization in cotton fiber development and …

GhKNL1 controls fiber elongation and secondary cell wall synthesis by repressing its downstream genes in cotton (Gossypium hirsutum)

Y Wang, Y Li, SY Gong, LX Qin, XY Nie… - Journal of Integrative …, 2022 - Wiley Online Library
… GhUBI1 was used as an internal control for normalization. (B) … of fiber elongation and SCW
synthesis to form the appropriate … of cotton genes was analyzed by qRT-PCR using a cotton

[HTML][HTML] Characterization of cotton ARF factors and the role of GhARF2b in fiber development

X Zhang, J Cao, C Huang, Z Zheng, X Liu… - BMC genomics, 2021 - Springer
… ) genes in cotton fiber development need be further explored. … in the overexpression lines
according to qRT-PCR analysis; for … hirsutum samples were normalized by calibrating with the …

[HTML][HTML] Comparative transcriptome analysis of fiber and nonfiber tissues to identify the genes preferentially expressed in fiber development in Gossypium hirsutum

J Yang, L Gao, X Liu, X Zhang, X Wang, Z Wang - Scientific Reports, 2021 - nature.com
… Identifying cotton fiber development-related genes and … was used for relative gene
expression normalization 24 . The … to the qRT-PCR and RNA-seq data, we found that the gene

[HTML][HTML] Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton

A Razzaq, MM Zafar, P Li, G Qun, X Deng… - Frontiers in Plant …, 2021 - frontiersin.org
… Therefore, CSSLs are considered to be the ideal material for … three transgenic cotton plants
was performed by qRT-PCR (… gene was used as an internal control for the normalization of …

Exploring the regulatory role of non-coding RNAs in fiber development and direct regulation of GhKCR2 in the fatty acid metabolic pathway in upland cotton

J Wang, X Wang, L Wang, MF Nazir, G Fu… - International Journal of …, 2024 - Elsevier
… to 0.9, we finally obtain an appropriate soft-threshold value of 14, … levels determined by
qRT-PCR were normalized to the … effectors, reporter and internal control vectors used in transient …

Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization in Agave sisalana (a CAM-plant) under abiotic stresses

MB Sarwar, Z Ahmad, BA Anicet, M Sajid… - … and molecular biology …, 2020 - Springer
… ranked as the least stable genes in pooled samples. Pairwise … housekeeping genes would
be adequate to normalize the … of the suitable candidate’s HKGs for accurate qRT-PCR data …