Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton
… Therefore, CSSLs are considered to be the ideal material for … three transgenic cotton plants
was performed by qRT-PCR (… gene was used as an internal control for the normalization of …
was performed by qRT-PCR (… gene was used as an internal control for the normalization of …
Exploring the regulatory role of non-coding RNAs in fiber development and direct regulation of GhKCR2 in the fatty acid metabolic pathway in upland cotton
J Wang, X Wang, L Wang, MF Nazir, G Fu… - International Journal of …, 2024 - Elsevier
… to 0.9, we finally obtain an appropriate soft-threshold value of 14, … levels determined by
qRT-PCR were normalized to the … effectors, reporter and internal control vectors used in transient …
qRT-PCR were normalized to the … effectors, reporter and internal control vectors used in transient …
Analysis of the MIR160 gene family and the role of MIR160a_A05 in regulating fiber length in cotton
G Liu, J Liu, W Pei, X Li, N Wang, J Ma, X Zang… - Planta, 2019 - Springer
… In the qRT–PCR reactions, the cotton U6 snRNA was used as the internal control for miRNA
… Expression levels of these genes were normalized by FPKM, and the pheatmap package …
… Expression levels of these genes were normalized by FPKM, and the pheatmap package …
An analysis of lncRNAs related to fiber quality and the discovery of their target genes in a Gossypium hirsutum line with Gossypium mustelinum introgression
B Wang, M Ji, H Fang, H Gu, TG Mehari, J Han… - … and Applied Genetics, 2024 - Springer
… design qRT-PCR-specific primers, using the Upland cotton actin gene as a control for
normalization … In this study, four genes related to cotton fiber quality development were identified …
normalization … In this study, four genes related to cotton fiber quality development were identified …
Comprehensive analysis of WOX genes uncovers that WOX13 is involved in phytohormone-mediated fiber development in cotton
P He, Y Zhang, H Liu, Y Yuan, C Wang, J Yu, G Xiao - BMC plant biology, 2019 - Springer
… genes during cotton fiber development. Gene expression data were obtained from qRT-PCR …
hirsutum ubiquitin gene GhUBQ7 was used to normalize the expression of the GhWOX …
hirsutum ubiquitin gene GhUBQ7 was used to normalize the expression of the GhWOX …
Identification and expression analysis of Tubulin gene family in upland cotton
B Chen, J Zhao, G Fu, X Pei, Z Pan, H Li… - Journal of Cotton …, 2021 - Springer
… qRT-PCR analysis revealed that 12 α-tubulin and 7 β-tubulin … of all values, then were
normalized with the log 2 (FPKM+ 1) … GhUBQ (GhA10G005800) gene as an internal control, and a …
normalized with the log 2 (FPKM+ 1) … GhUBQ (GhA10G005800) gene as an internal control, and a …
Transcriptome analysis and identification of genes associated with fruiting branch internode elongation in upland cotton
F Ju, S Liu, S Zhang, H Ma, J Chen, C Ge, Q Shen… - BMC plant biology, 2019 - Springer
… Our RNA-Seq and qRT-PCR results indicate that the … reads for each gene was then counted
and normalized into reads … for the selected genes and the internal control gene (UBQ) are …
and normalized into reads … for the selected genes and the internal control gene (UBQ) are …
Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis
Q Ma, N Wang, P Hao, H Sun, C Wang, L Ma, H Wang… - BMC plant biology, 2019 - Springer
… ) method was employed to normalize the total short read … cotton varieties with different fiber
quality by qRT-PCR. a The … large cotton varieties have also been performed, providing a good …
quality by qRT-PCR. a The … large cotton varieties have also been performed, providing a good …
The miR319-targeted GhTCP4 promotes the transition from cell elongation to wall thickening in cotton fiber
… Cotton fiber cells are developmentally synchronous, highly elongated, and contain nearly …
role in balancing cotton fiber cell elongation and wall synthesis. During fiber development the …
role in balancing cotton fiber cell elongation and wall synthesis. During fiber development the …
[HTML][HTML] Selection of stable housekeeping genes for qPCR in biotic and abiotic stressed jute and their application in lignin pathway analysis
S Parida, SK Datta, N Dey, S Majumder - Plant Stress, 2024 - Elsevier
… gene expression via qRT-PCR, a reliable housekeeping gene (HKG), also known as an internal
control gene … 18S housekeeping gene was employed for qRT-PCR data normalisation at …
control gene … 18S housekeeping gene was employed for qRT-PCR data normalisation at …