The comparative transcriptome and co-expression of hub genes analysis for fiber development in RIL populations of upland cotton
R Wei, Y Zhong, N Xu, P Li, MT Azhar, Z Yang… - Industrial Crops and …, 2024 - Elsevier
… However, fiber quality in upland cotton is not as good as that … The trend of qRT-PCR results
and RNA-seq results were … of the candidate genes determined by qRT-PCR, which was …
and RNA-seq results were … of the candidate genes determined by qRT-PCR, which was …
Data mining of transcriptional biomarkers at different cotton fiber developmental stages
… to the good quality and high yield verities of cotton which are … quantitative real-time PCR
(qRT-PCR) analysis in which they were … the normalization process, a total of 31,800 genes were …
(qRT-PCR) analysis in which they were … the normalization process, a total of 31,800 genes were …
GhbHLH18 negatively regulates fiber strength and length by enhancing lignin biosynthesis in cotton fibers
Z Gao, W Sun, J Wang, C Zhao, K Zuo - Plant Science, 2019 - Elsevier
… (A) qRT-PCR analysis indicates that GhbHLH18 gene expression is repressed in cotton
fibers of … The expression levels of GhbHLH18 were normalized to those of the internal control …
fibers of … The expression levels of GhbHLH18 were normalized to those of the internal control …
Overexpression of a sucrose synthase gene indirectly improves cotton fiber quality through sucrose cleavage
… SuS expression levels were normalized with GAPDH. For the … using qRT-PCR with 2 μl of
cDNA from the four transgenic cotton … cotton produced in this study is the best source for use in …
cDNA from the four transgenic cotton … cotton produced in this study is the best source for use in …
Identification and validation of reference genes for qRT-PCR analyses under different experimental conditions in Allium wallichii
Y Lin, G Liu, Y Rao, B Wang, R Tian, Y Tan… - Journal of Plant …, 2023 - Elsevier
… first set of suitable reference genes for qRT-PCR analyses, … one reference gene as internal
controls. The primers used for … genes that can be employed for qRT-PCR normalization. …
controls. The primers used for … genes that can be employed for qRT-PCR normalization. …
Insight into the relationship between S-lignin and fiber quality based on multiple research methods
S Zhang, T Jia, Z Zhang, X Zou, S Fan, K Lei… - Plant Physiology and …, 2020 - Elsevier
… transcriptome data was verified by qRT-PCR assay. Total 62 … quality during cotton fiber
development, we speculate that the ratio … ) gene was used as an internal control to normalize the …
development, we speculate that the ratio … ) gene was used as an internal control to normalize the …
Identification of key proteins related to high-quality fiber in Upland cotton via proteomics analysis
J Jiang, Z Shi, F Ma, K Liu - Plant Cell Reports, 2022 - Springer
… The relative transcript level was determined and normalized … best, followed by Sea island
cotton Pima 3–79 and Upland … in developing fibers of SY6167 and TM-1 using qRT-PCR. A …
cotton Pima 3–79 and Upland … in developing fibers of SY6167 and TM-1 using qRT-PCR. A …
Genome-Wide Identification and Characterization of GhCOMT Gene Family during Fiber Development and Verticillium Wilt Resistance in Cotton
C Wu, D Zuo, S Xiao, Q Wang, H Cheng, L Lv, Y Zhang… - Plants, 2021 - mdpi.com
… The data were normalized, and FPKM (Fragments per … From the results of qRT-PCR, we
can observe that the expression … GhUBQ7 (DQ116441) was used as the internal control. Each …
can observe that the expression … GhUBQ7 (DQ116441) was used as the internal control. Each …
Identification of Loci and Candidate Genes Responsible for Fiber Length in Upland Cotton (Gossypium hirsutum L.) via Association Mapping and Linkage Analyses
C Zhang, L Li, Q Liu, L Gu, J Huang, H Wei… - Frontiers in plant …, 2019 - frontiersin.org
… during the fiber development stage, and the qRT-PCR analysis … as an internal reference gene
to normalize the cDNA … in cotton is a highly polygenic trait and is suitable for QTL analyses. …
to normalize the cDNA … in cotton is a highly polygenic trait and is suitable for QTL analyses. …
Overexpression of cotton Trihelix transcription factor GhGT-3b_A04 enhances resistance to Verticillium dahliae and affects plant growth in Arabidopsis thaliana
H Mao, W Zhang, J Lv, J Yang, S Yang, B Jia… - Journal of Plant …, 2023 - Elsevier
… gene AtACTIN in Arabidopsis was used as an internal control… mapped (FPKM) normalized
log10 (transformed counts). The … We then performed qRT-PCR of some genes in the …
log10 (transformed counts). The … We then performed qRT-PCR of some genes in the …