Detection of candidate genes and development of KASP markers for Verticillium wilt resistance by combining genome-wide association study, QTL-seq and …

Y Zhao, W Chen, Y Cui, X Sang, J Lu, H Jing… - … and Applied Genetics, 2021 - Springer
… The cotton actin-7 gene was used as the internal control to normalize the variance among
… from the RNA-Seq in the resistant line was in agreement with the qRT-PCR, and the down-…

Genome-wide analysis elucidates the roles of GhHMA genes in different abiotic stresses and fiber development in upland cotton

C Wu, S Xiao, D Zuo, H Cheng, Y Zhang… - Plant Physiology and …, 2023 - Elsevier
… Transcriptomic data and qRT-PCR showed that GhHMA26 … All gene expression levels were
normalized by log 2 (FPKM+1). The heat … ) gene was used as the internal control. Relative …

[HTML][HTML] Identification of TPX2 Gene Family in Upland Cotton and its Functional Analysis in Cotton Fiber Development

K Lei, A Liu, S Fan, H Peng, X Zou, Z Zhen, J Huang… - Genes, 2019 - mdpi.com
… The authors used the GhHistone3 gene as an internal control, … the volume used to perform
qRT-PCR with three biological and … were normalized against the reference gene GhHistone3. …

Systematic analysis and comparison of CaLB genes reveal the functions of GhCaLB42 and GhCaLB123 in fiber development and abiotic stress in cotton

S Xiao, C Wu, D Zuo, H Cheng, Y Zhang, L Lv… - Industrial Crops and …, 2022 - Elsevier
qRT-PCR, and the predominant expression genes during … All gene expression levels were
normalized by log2 (FPKM+1). … (DQ116441) gene was used as the internal control. Relative …

Screening of abiotic stress‐responsive cotton genes using a cotton full‐length cDNA overexpressing Arabidopsis library

S Li, H Chen, Z Hou, Y Li, C Yang… - Journal of Integrative …, 2020 - Wiley Online Library
… Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,
qRT-PCR) and normalized by the 2 −ΔΔCt method using the UBQ gene as an internal control

[HTML][HTML] … cotton nonexpressor of pathogenesis-related 1 (NPR1)-like genes family: evolutionary analysis and putative role in fiber development and defense pathway

N Agarwal, R Srivastava, A Verma, KM Rai, B Singh… - Plants, 2020 - mdpi.com
… impact of GhNPR genes on fiber development, qRT-PCR was … Additionally, for the proper
development of the plant, its … Internal control gene GhUBQ4 was used to normalize the …

[HTML][HTML] Phenylpropanoid metabolism and pigmentation show divergent patterns between brown color and green color cottons as revealed by metabolic and gene …

Z Li, Q Su, M Xu, J You, AQ Khan, J Li, X Zhang… - Journal of Cotton …, 2020 - Springer
… the reference gene to normalize gene expression … qRT-PCR was used to detect the expression
levels of lignin and lignan biosynthetic pathway genes in these three types of cotton fibers

Cotton green fiber promotes suberin synthesis interfering cellulose deposition in the secondary cell wall

Z Chen, Y Li, Z Teng, Y Zhang, Y Liu, Q Suo… - Industrial Crops and …, 2023 - Elsevier
… ), cotton fiber is regarded as an ideal model for plant cell … Moreover, qRT-PCR analysis
indicated that Gh_D11G3168 … genes in Gossypium hirsutum for accurate normalization of …

[HTML][HTML] Over-expression of GhACTIN1 under the control of GhSCFP promoter improves cotton fiber and yield

A Iqbal, S Aslam, S Akhtar, Q Ali, AQ Rao… - Scientific Reports, 2023 - nature.com
Cotton fiber provides a good model for studying cell elongation … Data normalization was
done using GAPDH as internal control … . The qRT-PCR analysis in advance generation (T 1 ) at …

GhERF41, a group IIIe ERF protein, participates in regulating cell wall synthesis in cotton fiber

Y Gao, Y Cui, S Li, M Feng, L Liu, W Xu… - Industrial Crops and …, 2023 - Elsevier
genes in fiber development, we examined their spatiotemporal expression in different tissues
at various developmental stages via qRT-PCR… as the internal control for normalization. …