Detection of candidate genes and development of KASP markers for Verticillium wilt resistance by combining genome-wide association study, QTL-seq and …
Y Zhao, W Chen, Y Cui, X Sang, J Lu, H Jing… - … and Applied Genetics, 2021 - Springer
… The cotton actin-7 gene was used as the internal control to normalize the variance among
… from the RNA-Seq in the resistant line was in agreement with the qRT-PCR, and the down-…
… from the RNA-Seq in the resistant line was in agreement with the qRT-PCR, and the down-…
Genome-wide analysis elucidates the roles of GhHMA genes in different abiotic stresses and fiber development in upland cotton
C Wu, S Xiao, D Zuo, H Cheng, Y Zhang… - Plant Physiology and …, 2023 - Elsevier
… Transcriptomic data and qRT-PCR showed that GhHMA26 … All gene expression levels were
normalized by log 2 (FPKM+1). The heat … ) gene was used as the internal control. Relative …
normalized by log 2 (FPKM+1). The heat … ) gene was used as the internal control. Relative …
[HTML][HTML] Identification of TPX2 Gene Family in Upland Cotton and its Functional Analysis in Cotton Fiber Development
K Lei, A Liu, S Fan, H Peng, X Zou, Z Zhen, J Huang… - Genes, 2019 - mdpi.com
… The authors used the GhHistone3 gene as an internal control, … the volume used to perform
qRT-PCR with three biological and … were normalized against the reference gene GhHistone3. …
qRT-PCR with three biological and … were normalized against the reference gene GhHistone3. …
Systematic analysis and comparison of CaLB genes reveal the functions of GhCaLB42 and GhCaLB123 in fiber development and abiotic stress in cotton
S Xiao, C Wu, D Zuo, H Cheng, Y Zhang, L Lv… - Industrial Crops and …, 2022 - Elsevier
… qRT-PCR, and the predominant expression genes during … All gene expression levels were
normalized by log2 (FPKM+1). … (DQ116441) gene was used as the internal control. Relative …
normalized by log2 (FPKM+1). … (DQ116441) gene was used as the internal control. Relative …
Screening of abiotic stress‐responsive cotton genes using a cotton full‐length cDNA overexpressing Arabidopsis library
S Li, H Chen, Z Hou, Y Li, C Yang… - Journal of Integrative …, 2020 - Wiley Online Library
… Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,
… qRT-PCR) and normalized by the 2 −ΔΔCt method using the UBQ gene as an internal control…
… qRT-PCR) and normalized by the 2 −ΔΔCt method using the UBQ gene as an internal control…
[HTML][HTML] … cotton nonexpressor of pathogenesis-related 1 (NPR1)-like genes family: evolutionary analysis and putative role in fiber development and defense pathway
… impact of GhNPR genes on fiber development, qRT-PCR was … Additionally, for the proper
development of the plant, its … Internal control gene GhUBQ4 was used to normalize the …
development of the plant, its … Internal control gene GhUBQ4 was used to normalize the …
[HTML][HTML] Phenylpropanoid metabolism and pigmentation show divergent patterns between brown color and green color cottons as revealed by metabolic and gene …
Z Li, Q Su, M Xu, J You, AQ Khan, J Li, X Zhang… - Journal of Cotton …, 2020 - Springer
… the reference gene to normalize gene expression … qRT-PCR was used to detect the expression
levels of lignin and lignan biosynthetic pathway genes in these three types of cotton fibers …
levels of lignin and lignan biosynthetic pathway genes in these three types of cotton fibers …
Cotton green fiber promotes suberin synthesis interfering cellulose deposition in the secondary cell wall
Z Chen, Y Li, Z Teng, Y Zhang, Y Liu, Q Suo… - Industrial Crops and …, 2023 - Elsevier
… ), cotton fiber is regarded as an ideal model for plant cell … Moreover, qRT-PCR analysis
indicated that Gh_D11G3168 … genes in Gossypium hirsutum for accurate normalization of …
indicated that Gh_D11G3168 … genes in Gossypium hirsutum for accurate normalization of …
[HTML][HTML] Over-expression of GhACTIN1 under the control of GhSCFP promoter improves cotton fiber and yield
… Cotton fiber provides a good model for studying cell elongation … Data normalization was
done using GAPDH as internal control … . The qRT-PCR analysis in advance generation (T 1 ) at …
done using GAPDH as internal control … . The qRT-PCR analysis in advance generation (T 1 ) at …
GhERF41, a group IIIe ERF protein, participates in regulating cell wall synthesis in cotton fiber
Y Gao, Y Cui, S Li, M Feng, L Liu, W Xu… - Industrial Crops and …, 2023 - Elsevier
… genes in fiber development, we examined their spatiotemporal expression in different tissues
at various developmental stages via qRT-PCR… as the internal control for normalization. …
at various developmental stages via qRT-PCR… as the internal control for normalization. …