GhERF41, a group IIIe ERF protein, participates in regulating cell wall synthesis in cotton fiber

Y Gao, Y Cui, S Li, M Feng, L Liu, W Xu… - Industrial Crops and …, 2023 - Elsevier
genes in fiber development, we examined their spatiotemporal expression in different tissues
at various developmental stages via qRT-PCR… as the internal control for normalization. …

Genome-wide association study of micronaire using a natural population of representative upland cotton (Gossypium hirsutum L.)

J Song, W Pei, J Ma, S Yang, B Jia, Y Bian… - Journal of Cotton …, 2021 - Springer
… a genetic reference for genetic improvement of cotton fiber micronaire … In this study, qRT-PCR
was used to determine the … for high yarn strength and good spinning quality of cotton fiber. …

GhEXL3 participates in brassinosteroids regulation of fiber elongation in Gossypium hirsutum

C Zhang, Z Liu, S Shu, X Li, Y Li, L Liu, L Liu… - The Plant … - Wiley Online Library
… We utilized qRT-PCR analysis to examine the expression … The scale bar represents the
Z-score normalized fragment per … as internal controls in the PCR experiments of cotton and …

[HTML][HTML] Uncloaking lncRNA-meditated gene expression as a potential regulator of CMS in cotton (Gossypium hirsutum L.)

R Hamid, F Jacob, H Marashi, V Rathod, RS Tomar - Genomics, 2020 - Elsevier
… of lncRNAs in regulating lignin metabolism in cotton fibers via miR397 [18]. … The internal
control gene used for normalization was the … The details of qRT-PCR primers used in this study …

Identification and characterization of circular RNAs involved in the fertility stability of cotton CMS-D2 restorer line under heat stress

R Wang, M Zhang, H Wang, L Chen, X Zhang, L Guo… - BMC Plant …, 2024 - Springer
… by RT-PCR and qRT-PCR. Functional enrichment and metabolic … increase cotton yield, but
also improve fiber quality and … a linear control and internal reference gene for normalization. …

An easy and rapid transformation protocol for transient expression in cotton fiber

X Shang, L Zhu, Y Duan, Q He, M Zhao, Y Yu… - Frontiers in Plant …, 2022 - frontiersin.org
… that exogenous genes could be expressed in cotton fibers via … transcript levels quantified by
qRT-PCR in cotton fibers (Figure 4A)… was suitable for promoter characterization in fiber cells. …

Fine-mapping and candidate gene analysis of qFS-Chr. D02, a QTL for fibre strength introgressed from a semi-wild cotton into Gossypium hirsutum

L Feng, C Zhou, Q Su, M Xu, H Yue, S Zhang, B Zhou - Plant Science, 2020 - Elsevier
… All data were normalized to the expression level of actin. * P … lines (NILs), are an ideal tool
for precise QTL mapping of complex … qRT-PCR and SNP analyses revealed seven genes were …

Profiling of MicroRNAs Involved in Mepiquat Chloride-Mediated Inhibition of Internode Elongation in Cotton (Gossypium hirsutum L.) Seedlings

L Wang, Y Yin, X Jing, M Wang, M Zhao, J Yu… - Frontiers in Plant …, 2021 - frontiersin.org
… (Gossypium hirsutum L.) is a worldwide cultivated economic crop that provides natural fiber
… The U6 gene was used as an internal control for the normalization of qRT-PCR data. The …

Cotton microtubule-associated protein GhMAP20L5 mediates fiber elongation through the interaction with the tubulin GhTUB13

Q Song, W Gao, C Du, J Wang, K Zuo - Plant science, 2023 - Elsevier
… of all the values, then were normalized with the log2 (FPKM+1… qRT-PCR results showed
that the expression level of … Ubiquitin gene (UBI) was used as an internal control. Error …

GhODO1, an R2R3-type MYB transcription factor, positively regulates cotton resistance to Verticillium dahliae via the lignin biosynthesis and jasmonic acid signaling …

Y Zhu, X Hu, P Wang, H Wang, X Ge, F Li… - International Journal of …, 2022 - Elsevier
… economic value of renewable textile fibers. Notably, upland … gene to normalize the gene
expression, and the relative … -PCR and qRT-PCR in TRV:00 and GhODO1-silenced cotton plants …