Synchronous transcription of cytoskeleton-associated genes is critical to cotton fiber elongation

DK Pandey, B Chaudhary - Journal of Plant Growth Regulation, 2019 - Springer
Normalization of relative gene expression data was … GAPDH gene as an internal reference
for different cotton tissues (… in the developing cell wall is required for proper reorganization of …

[HTML][HTML] Transcriptomic analysis of gene expression of Verticillium dahliae upon treatment of the cotton root exudates

X Zhang, W Cheng, Z Feng, Q Zhu, Y Sun, Y Li, J Sun - BMC genomics, 2020 - Springer
… dahliae gene were generated and normalized to FPKM (… gene specific primers used in
qRT-PCR are listed in Table 1, and the V. dahliae tubulin gene was used as an internal control

[PDF][PDF] Journal of Bioresource Management

M Nadeem, N Iqbal, U Waheed, ZU Zia, M Ali - academia.edu
… of cotton fiber development and was reverse transcribed to cDNA for PCR amplification. For
data normalization, 18s rRNA was used as an internal control. The … The qRT-PCR assay was …

… of the transcriptome and metabolome reveals the role of the flavonoid biosynthesis pathway in regulating axillary buds in upland cotton (Gossypium hirsutum L.)

J Shi, H Zhou, X Liu, N Wang, Q Xu, G Yan - Planta, 2021 - Springer
… b Correlation analysis of the gene expression ratios between qRT-PCR and … cotton UBQ7
gene, as an internal control, was used to normalize the expression levels of the target genes. …

[HTML][HTML] Differential transcription and alternative splicing in cotton underly specialized defense responses against pests

DY Chen, QY Chen, DD Wang, YP Mu… - Frontiers in plant …, 2020 - frontiersin.org
Cotton is a global important fiber crop. The green mirid bug (… The FPKM of each gene
was normalized by z-score. The … The expression of indicated genes was detected by qRT-PCR. …

Temporal expression profiling of GhNAC transcription factor genes in cotton cultivars under abiotic stresses

S Sivakumar, GP Kumar, S Vinoth, G Siva… - Plant Gene, 2021 - Elsevier
… of natural fibers used in the fabric production contributing … after 1 h and 12 h stress treatments
for qRT-PCR analysis. … along with the target gene to normalize the expression among …

Large‐fragment insertion activates gene GaFZ (Ga08G0121) and is associated with the fuzz and trichome reduction in cotton (Gossypium arboreum)

X Wang, Y Miao, Y Cai, G Sun, Y Jia… - Plant Biotechnology …, 2021 - Wiley Online Library
… -based cloning and qRT-PCR, the recessive fuzzless gene n 2 … ) from cotton was selected
as an internal control. The relative … Protein concentration was used to normalize GUS activity. …

[HTML][HTML] Two Aquaporin Genes, GhPIP2;7 and GhTIP2;1, Positively Regulate the Tolerance of Upland Cotton to Salt and Osmotic Stresses

A Guo, J Hao, Y Su, B Li, N Zhao, M Zhu… - Frontiers in Plant …, 2022 - frontiersin.org
… was used as the internal control to calculate and normalize the … To verify their expression
levels, we performed qRT-PCR (… However, to the best of our knowledge, studies focused on the …

Transcriptome analysis reveals differentially expressed ERF transcription factors associated with salt response in cotton

L Long, WW Yang, P Liao, YW Guo, A Kumar, W Gao - Plant science, 2019 - Elsevier
… as the reference to normalize genes expression levels. The … time points, we performed
qRT-PCR to analyse the transcripts of … : DQ116441 ) gene was used as an internal control. Error …

Genome-wide identification of cotton GRAM family proteins reveals that GRAM31 regulates fiber length

Z Ye, L Qiao, X Luo, X Chen, X Zhang… - Journal of Experimental …, 2021 - academic.oup.com
… ovule through cell differentiation, and provides a good experimental system to … fiber
development gene network in cotton. Relative expression was determined using qRT-PCR for genes