Transcriptome profiling provides New insights into the molecular mechanism underlying the sensitivity of cotton varieties to Mepiquat Chloride

Z Wang, Y Li, Q Zhu, L Tian, F Liu, X Zhang… - International Journal of …, 2022 - mdpi.com
… (DQ116441) was used as the internal control. The qRT-PCR assays were performed in
96-well … The relative gene expression level was calculated and normalized using the 2 − ΔΔCT …

Cotton plant defence against a fungal pathogen is enhanced by expanding BLADE-ON-PETIOLE1 expression beyond lateral-organ boundaries

Z Zhang, P Wang, X Luo, C Yang, Y Tang… - Communications …, 2019 - nature.com
… To obtain the best experience, we recommend you use a more … RT/qRT-PCR was employed
to monitor related genes … and GhUb-7 gene were used as internal control for normalization, …

Transcriptomic analysis reveals the key role of histone deacetylation via mediating different phytohormone signalings in fiber initiation of cotton

Z Wei, Y Li, F Ali, Y Wang, J Liu, Z Yang, Z Wang… - Cell & Bioscience, 2022 - Springer
… key role in the fiber cell initiation through the proper spatiotemporal … The qRT-PCR for each
gene was performed in three … expression value was quantified and normalized to the value of …

Cotton ethylene response factor GhERF91 involved in the defense against Verticillium dahliae

A Nurimanguli, Y WU, Z PAN, Q AN, G SHUI… - Journal of Integrative …, 2023 - Elsevier
… A normalized gene expression matrix was used as the input, and 36 transcriptome samples
were … C, qRT-PCR analysis of GhERF91 in cotton with or without (mock) ethylene (5 mg L-1) …

[HTML][HTML] Fiber-specific increase of carotenoid content promotes cotton fiber elongation by increasing abscisic acid and ethylene biosynthesis

J Zeng, D Yao, M Luo, L Ding, Y Wang, X Yan, C Wang… - The Crop Journal, 2023 - Elsevier
qRT-PCR indicated that the transcriptional level of GhOR1 … concentration to a level more
suitable for fiber elongation. … GhHis3 and GhUbiquitin served as internal controls. Error bars …

Comprehensive genome-wide analysis of thaumatin-like gene family in four cotton species and functional identification of GhTLP19 involved in regulating tolerance to …

Z Li, X Wang, Y Cui, K Qiao, L Zhu, S Fan… - Frontiers in Plant …, 2020 - frontiersin.org
… , biotic stress and cotton fiber development. GhTLP19 on … were normalized using cotton
ubiquitin 7 (UBQ7) as an internal … Therefore, we also used public RNA-seq and qRT-PCR data …

… of candidate genes, Gohir. D05G103700 and Gohir. D12G153600, identified through expression QTL analysis using virus-induced gene silencing in upland cotton  …

S Naveed, S Rustgi - Agriculture, 2023 - mdpi.com
… The cotton ACTIN1 gene was used as an internal control for … the treatment for RNA extraction
and qRT-PCR analysis. The … was used for the normalization of the gene expression data. …

Genome-wide identification of xylan glucuronosyltransferase family in cotton and function characterization of GhGUX5 in regulating Verticillium wilt resistance

L Zhang, Y Yu, M Zhang, K Rong, Y Wu… - International Journal of …, 2023 - Elsevier
… RNA-Seq data and qRT-PCR analysis both indicated that … Cotton is an important source of
natural fiber and vegetable oils, … FPKM values for gene expression were log2 normalized and …

The lignin riddle in jute: A comparative genomic investigation identifies targets for improving fiber quality

MS Hossain, B Ahmed, R Ahmed, MW Ullah… - Gene Reports, 2024 - Elsevier
… Bangladesh imported 82.8 lakh bales of cotton of nearly $3 billion … qRT-PCR to identify
the core lignification genes involved in secondary cell wall formation during fiber development. …

… -wide characterization and expression analysis of geranylgeranyl diphosphate synthase genes in cotton (Gossypium spp.) in plant development and abiotic stresses

F Ali, G Qanmber, Z Wei, D Yu, Y Li, L Gan, F Li… - BMC genomics, 2020 - Springer
… The relative expression was normalized to the CK (0 h) … selected GhGGPPS genes by
qRT-PCR. The GhGGPPS … Bank, accession number AF024716) was used as an internal control [53…