… of each ionizable residue in the protein. • Residues that are … Mutant obtained by replacing
a destabilizing charged residue … acid residues to be mutated using the TKSA-MC web server. …

… proteins to their thermal stability using the TKSA-MC, a Web server for rational mutation via
optimizing the protein charge … The TKSA-MC is a Web server developed by our research …

… We propose to perform rational residue … are optimized using the TKSA-MC web server to
determine destabilizing residues in the WT to serve as candidates to be experimentally mutated. …

… Mutation candidates indicated by the TKSA-MC web server … TKSA-MC: a web server
for rational mutation through the … One of the advantages of optimizing the protein charge-charge …

… mutants of PspAG97A using a free web server that calculates protein charge-charge interactions
via the TKSA … successful application of rational design guided by the TKSA-MC model in …

… proteins due to mutations have been developed during the last 30 years, and many different
web servers … to calculate the effect of mutations on the net charge–charge interaction; ≤ 150 …

… (A) The charge-charge interaction energy (ΔGqq) calculated by the TKSA-MC model for each
… also useful guidance for their rational optimisation for efficient and precise genome editing. …

… method, Bayesian Optimization-guided EVOlutionary (BO-… to explore protein fitness landscapes
of combinatorial mutagenesis … specificity of RhlA through either (semi-)rational design or …

… , we have collected an experimental dataset of nine highly mutated proteins from FireProt …
web server and the charge interactions can be optimized using the methods like TKSA-MC [101…

… engineer protein stabilities through mutations involving charged … in semi-rational protein
design to give improved protein stability, … TKSA-MC [19] and pStab [20] tools tackle this issue by …