… activating enzyme 5 (UBA5) activates UFM1 through an adenylation reaction that consumes
ATP. UFM1-conjugase 1 (UFC1), the E2 conjugating enzyme, retrieves UFM1 from UBA5 by …

… enzymatic reaction involving E1, the UFM1-activating enzyme (ubiquitin-like modifier-activating
enzyme 5, UBA5… For instance, Uba5 knockout mice exhibited severe anemia, followed by …

… Mouse genetics studies also revealed the indispensable roles of this … essential for conjugation.
The mature UFM1 is activated by the homodimer of UBA5, a UFM1-specific E1 enzyme, …

… , UBA5, UFL1 and UFBP1, leads to defective erythropoiesis … In adult mice, acute ablation of
either UFL1 or UFBP1 causes … indispensable role of UFMylation in definitive erythropoiesis [7…

… While the UFM1 activating and conjugating enzymes share … of the E1 enzyme (UBA5) at the
apex of the enzymatic cascade is … In particular, germline deletion of UBA5 in mice invoked in …

… -specific enzymes, namely, Ufm1-activating E1 enzyme Uba5, … the indispensable role of
ufmyation in erythropoiesis 20,21,22 . … Ufl1 and Ufbp1 conditional knockout mice, we frequently …

… exposes the glycine residue essential for conjugation 3 . … -energy thioester bond with UBA5,
a UFM1-specific E1 enzyme 1 . … defective erythroid differentiation and neurogenesis in mice 7…

… action of three enzymes: a UFM1-activating E1, a … erythroid progenitors is observed in
UBA5 KO embryos. In line with their essential roles in UFMylation, UFL1 and DDRGK1 KO mice …

… Ufm1 is then activated by the E1-activating enzyme Uba5 … mouse bone marrow without
UFL1 and UFBP1, the percentage of diversified cell types showed some variance, and erythroid …

… The UFM1-activating enzyme, UBA5, possesses two isoforms: long and short. These … are
not essential for UBA5 function, therefore raising the question of their role in UBA5's function. …