Fluorescence Anisotropy: Probing Rotational Dynamics of Biomolecules

GP Pattnaik, H Chakraborty - Optical Spectroscopic and Microscopic …, 2022 - Springer
… -state and time-resolved fluorescence anisotropy and their … -state fluorescence anisotropy of
a molecular rotor, BODIPY-C 12 [30]… with depletion of cholesterol (Fig. 4.8). The fluorescence …

Prediction of paclitaxel pharmacokinetic based on in vitro studies: Interaction with membrane models and human serum albumin

AM Carvalho, E Fernandes, H Gonçalves… - International journal of …, 2020 - Elsevier
… light scattering, fluorescence anisotropy, atomic force … by steady-state/time-resolved
fluorescence and computer … black) and time-resolved fluorescence (τ 0 /τ in red) of the 12-AS …

[HTML][HTML] Assessing the role of membrane lipids in the action of ruthenium (III) anticancer compounds

R Starosta, TC Santos, AF Dinis de Sousa… - Frontiers in molecular …, 2023 - frontiersin.org
… , sphingomyelin and cholesterol were used to mimic the lipid … and anisotropy, was
performed using the Time-Resolved … A fluorescence quenching study using n-(9-anthroyloxy)-…

[HTML][HTML] Tocopheryl succinate-induced structural changes in DPPC liposomes: DSC and ANS fluorescence studies

G Neunert, J Tomaszewska-Gras, S Witkowski… - Molecules, 2020 - mdpi.com
… Based on the fluorescence and anisotropy methods, Mason … ANS steady-state and time-resolved
emission spectroscopy … is modulated by the presence of cholesterol in a concentration-…

[HTML][HTML] What does time-dependent fluorescence shift (TDFS) in biomembranes (and proteins) report on?

F Scollo, H Evci, M Amaro, P Jurkiewicz… - Frontiers in …, 2021 - frontiersin.org
… present an anisotropic system … of cholesterol (chol) on the calcium adsorption to PC/PS
model membranes. The results include the position of the maxima of Time-Resolved Emission

Solubilization in Micelles and Vesicles Studied by Fluorescence Techniques: Interplay Between the Microproperties of the Aggregates and the Locus and Extent of …

E Lissi, E Abuin - Solubilization in Surfactant Aggregates, 2020 - taylorfrancis.com
… from a kinetic analysis of time-resolved fluorescence data [14]. … from the effect that they
produce upon the anisotropy of DPH (l,… (9-anthroyloxy)fatty acids in DODAC giant vesicles [89] or …

[HTML][HTML] Assessment of DPPC Liposome Disruption by Embedded Tocopheryl Malonate

G Neunert, J Tomaszewska-Gras… - Applied Sciences, 2023 - mdpi.com
… of phospholipids in a similar way as cholesterol [7]. Due to Toc … , emission wavelength,
and fluorescence anisotropy of … The samples were stored at 0–4 C for at least 12 h before the …

Unfolding the role of a flavone-based fluorescent antioxidant towards the misfolding of amyloid proteins: An endeavour to probe amyloid aggregation

A Karmakar, T Mallick, C Fouzder… - The Journal of …, 2020 - ACS Publications
… , steady-state and time-resolved fluorescence spectroscopy, fluorescence anisotropy, 1 H …
g were conditioned at 25 ± 2 C with a 12 h light/12 h dark cycle. Mice were divided into two …

Liposomes as biomembrane models: Biophysical techniques for drug-membrane interaction studies

S Andrade, MJ Ramalho, JA Loureiro… - Journal of molecular …, 2021 - Elsevier
… composed of DMPC, DMPC:cholesterol and DMPC:cholesterol:SM, and labelled with DPH …
state and time-resolved events. This method is based on the principle of anisotropy that relies …

Study on assembling compactness of amphiphilic calixarenes by fluorescence anisotropy

HW Tian, Z Xu, HB Li, XY Hu, DS Guo - Supramolecular Chemistry, 2021 - Taylor & Francis
… The maximum fluorescence intensities were obtained after 12 h of stirring at room …
anisotropies (r) of DPH ( Table 1 ). It is noteworthy that the direct comparison of anisotropies for …