Allostery in the nitric oxide dioxygenase mechanism of flavohemoglobin

AM Gardner, PR Gardner - Journal of Biological Chemistry, 2021 - ASBMB
… activate the NO dioxygenase function of Escherichia coli flavohemoglobin. Steady-state and
E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …

Ordered Motions in the Nitric-Oxide Dioxygenase Mechanism of Flavohemoglobin and Assorted Globins with Tightly Coupled Reductases

PR Gardner - Protein Reviews: Volume 23, 2022 - Springer
… -detoxifying NO metabolic activity with the flavoHb in Escherichia coli (Gardner et al. 1998a;
… modeled on the E. coli ferric flavoHb with a bridging Coulomb blockade water molecule (W) …

Dioxygen and glucose force motion of the electron-transfer switch in the iron (III) flavohemoglobin-type nitric oxide dioxygenase

AM Gardner, PR Gardner - Journal of Inorganic Biochemistry, 2023 - Elsevier
… of the Escherichia coli flavoHb-NOD [8,9]. However, in the resting state, the Fe(III)-flavoHb
binds … and the bound ‘Coulomb blockade’ water molecule poised between the FAD C8M and …

Molecular characterization and expression profiling of two flavohemoglobin genes play essential roles in dissolved oxygen and NO stress in Saitozyma podzolica …

Q Yang, Y Ran, S Guo, F Li, D Xiang, Y Cao… - International Journal of …, 2023 - Elsevier
… Hence, the Fhb, also known as NO-dioxygenase, can … in an ice bath and a boiling water bath
three times for 10 min. … washed twice with 1 mL of distilled water, and re-suspended in 1 mL …

[HTML][HTML] Flavohaemoglobin: The pre-eminent nitric oxide–detoxifying machine of microorganisms

RK Poole - F1000Research, 2020 - ncbi.nlm.nih.gov
… It is soluble in water (approximately 1.6 mM at 37 C and 1.94 … to the NO dioxygenase
activities of flavohaemoglobin 12 . … Thus, for example, the Escherichia coli flavohaemoglobin (…

Expressed Soybean Leghemoglobin: Effect on Escherichia coli at Oxidative and Nitrosative Stress

OV Kosmachevskaya, EI Nasybullina, KB Shumaev… - Molecules, 2021 - mdpi.com
… , NO-dioxygenase, and peroxynitrite isomerase can play a pivotal role in the overall … the
synthesis of flavohemoglobin which plays a key role in protecting bacterial cells against NO [74]. …

A nitric oxide synthase–like protein from Synechococcus produces NO/NO3− from l-arginine and NAPDH in a tetrahydrobiopterin-and Ca2+-dependent manner

AL Picciano, BR Crane - Journal of Biological Chemistry, 2019 - ASBMB
Escherichia coli strains against nitrosative stress, even in a mutant devoid of NO-protective
flavohemoglobin… NOS and NO oxygenase activities, requires H 4 B, and may play a role in Ca …

[HTML][HTML] A critical role for staphylococcal nitric oxide synthase in controlling flavohemoglobin toxicity

RM Singh, SS Chaudhari, S Panda, EH Hutfless… - Redox Biology, 2023 - Elsevier
… Under these conditions, growth can only resume once nitric oxide (NO) is detoxified by the
flavohemoglobin Hmp. Paradoxically, S. epidermidis produces endogenous NO through its …

Impact of azole drugs on energetics, kinetics, and ligand migration pathways of CO photo-dissociation in bacterial flavohemoglobins

D Butcher, M Moussaoui, L Baciou, J Miksovska - RSC advances, 2020 - pubs.rsc.org
… fHbs exhibit a nitric oxide dioxygenase (NOD) activity which … of electron transfer in
flavohemoglobins, specifically disruption of the … groups through adjacent structural water

Giardia duodenalis: Flavohemoglobin is involved in drug biotransformation and resistance to albendazole

…, R Argüello-García, C Vázquez, E Saavedra… - PLoS …, 2022 - journals.plos.org
… Trophozoites were harvested by chilling the tubes in an iced water bath for 1 h. Detached …
electron shuttles, and one flavohemoglobin (gFlHb) with nitric oxide dioxygenase activity [14,33…