Direct measurement of pregnanediol 3-glucuronide (PDG) in dried urine spots by liquid chromatography-mass spectrometry to detect ovulation

DJ Handelsman, R Nimmagadda, R Desai… - The Journal of Steroid …, 2021 - Elsevier
… The sensitivity (limit of detection (LOD) and limit of quantitation (LOQ) defined according to …
3 levels (accuracy and CV%) and extraction recovery (6 replicates at 3 levels on pooled urine

Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC–MS/MS

J Chen, H Hou, H Chen, Y Luo, Y He, H Wang… - … and biomedical analysis, 2020 - Elsevier
… and urine are frequently chosen as samples for clinical steroid hormone analysis, because of
their non-invasion (urine) … Blood/serum and urine are integrated biological fluids, which can …

[HTML][HTML] Evaluating urinary estrogen and progesterone metabolites using dried filter paper samples and gas chromatography with tandem mass spectrometry (GC–MS …

M Newman, SM Pratt, DA Curran, FZ Stanczyk - BMC chemistry, 2019 - Springer
… metabolites from small volumes of dried urine using gas … precise quantification at the
lower concentrations of urinaryPregnanediol may exist as a glucuronide at the 3 position, at …

Targeted LC–MS/MS analysis of steroid glucuronides in human urine

R Wang, MF Hartmann, SA Wudy - The Journal of Steroid Biochemistry and …, 2021 - Elsevier
… Only a very little amount of unconjugated testosterone was found in older children. The only
… for quantification of steroid glucuronides in human urine. The method was applied to urinary

Longitudinal evaluation of multiple biomarkers for the detection of testosterone gel administration in women with normal menstrual cycle

O Salamin, R Nicoli, T Langer, J Boccard… - … and Analysis, 2022 - Wiley Online Library
… In females, T is produced in small amounts by the ovaries … in low urinary T levels, often
below the limit of quantification (… reference compounds (ERC) pregnanediol (PD) and 16-…

[HTML][HTML] Targeted metabolic profiling of urinary steroids with a focus on analytical accuracy and sample stability

N Vogg, T Müller, A Floren, T Dandekar… - Journal of Mass …, 2022 - Elsevier
… A method for the quantification of 11 deconjugated steroids (5-… , pregnanediol, and 5-pregnenediol)
in human urine was … for the quantification of a panel of 11 urinary steroids. We …

[HTML][HTML] A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine …

J Lin, S Li, B Ye, W Zheng, H Wang, Y Liu… - … in Bioengineering and …, 2023 - frontiersin.org
detection. The current colloidal gold strips mostly detect ovulation by setting a detection
The method developed in this paper achieved quantitative detection by using a fluorescent …

[HTML][HTML] Reference intervals for the urinary steroid metabolome: The impact of sex, age, day and night time on human adult steroidogenesis

D Ackermann, M Groessl, M Pruijm, B Ponte, G Escher… - PloS one, 2019 - journals.plos.org
… In humans, the small number of circulating sex steroids, … in S3 Table, with the exception of
pregnanediol (206 vs. 204 μg/24 … We quantified 40 urinary steroid metabolites by GC-MS in a …

Quantification of urinary steroids by supported liquid extraction with GC-MS/MS: Unravelling cyclic fluctuations of steroid profiling in regular menstrual cycle

M Liu, Y Ge, X Xu, L Liao - … of Pharmaceutical and Biomedical Analysis, 2022 - Elsevier
… The LLOQ was the lowest amount of an analyte that could be quantitatively determined
with acceptable precision (RSD ≤ 20%) and accuracy (recoveries between 80% and 120%). …

[HTML][HTML] Development of the thin film solid phase microextraction (TF-SPME) method for metabolomics profiling of steroidal hormones from urine samples using LC …

W Struck-Lewicka, B Karpińska, W Rodzaj… - Frontiers in Molecular …, 2023 - frontiersin.org
… The sensitivity enhancement is crucial in terms of extraction of trace amounts of compounds
or when there is a need for extraction of compounds at different concentration levels from …