… carried out with Q5 ® Site-directed mutagenesis kit (New England … However, although we
have the X-ray structures of the … From our crystallographic structures, mutagenesis, NMR and …

… 3B) and diffracted to a 1.8-Å resolution on an in-house X-ray source. An amino acid … to being
sequence verified using forward and reverse primers. Site-directed mutagenesis of HEV 510…

… , Biochemical, Site-directed mutagenesis (SDM), X-ray crystallographic and cryo-… , Site-directed
mutagenesis, X-ray crystallographic and cryo-EM data of these enzymes from RNA viral …

… In this study, a CTP-derived S 3 RdRP elongation complex (EC) of enterovirus 71 (EV71),
a close relative of PV, was captured by X-ray crystallography. Besides a previously identified …

… in single-stranded RNA recognition. Indeed, a subsequent Ec Hfq … An early study using
analytical ultracentrifugation (AUC), electrophoretic mobility shifts, and site-directed mutagenesis …

… polymerases, we report six X-ray structures of these variants, … as cloning, PCR, site-directed
mutagenesis, or sequencing. In … In summary, structural analysis of the crystal structure of an …

… We solved the crystal structure of SBD Hga complexed with … Site-directed mutagenesis was
carried out by whole-plasmid … An X-ray diffraction data set at 1.8 Å resolution was collected …

… RNA recognition and hydrogen-bond network in pockets 1 and 4. This is in perfect agreement
with our crystal structure… the X-ray structure were utilized to obtain starting structures where …

… Site-directed mutagenesis was used to obtain variants of this plasmid (Table 2), which were
… from X-ray data that is among the highest resolution data ever reported for an RNA of this …

… Site-directed mutagenesis (SDM) analysis and X-ray … ) and many DNAdependent RNA
polymerases (DdRps). The X-ray crystallographic structures of the complete heterotrimeric viral …