Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy

M Rubessa, ME Kandel, S Schreiber… - Systems Biology in …, 2020 - Taylor & Francis
… chose spatial light interference microscopy (SLIM), which is highly sensitive in both space and
… SLIM has been used recently to characterized the topography and refractometry of sperm (…

Spatial light interference microscopy: principle and applications to biomedicine

X Chen, ME Kandel, G Popescu - Advances in optics and photonics, 2021 - opg.optica.org
… the spatial accuracy of SLIM and atomic force microscopy (AFM) by imaging an amorphous
carbon film deposited on glass. The topography … of the sperm cell yield high refractive-index v…

Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress condition

V Dubey, D Popova, A Ahmad, G Acharya, P Basnet… - Scientific reports, 2019 - nature.com
… very low refractive index contrast with respect to the medium like tail of the sperm cells. …
investigation thus holography provides a 3D topographic profile of the specimen. The phase …

3D dynamic observation of human sperm by parallel phase-shifting digital holographic microscopy based on pixelated polarization

C Bai, Z Jiang, J Zhao, S Wu, Q Zhang - APL Photonics, 2023 - pubs.aip.org
… -magnification microscope objective, while the sperm flagella (… Microscopic dynamic
observation of intact sperm in 3D is … -dimensional refractive-index profile of the sperm head and …

… of bovine spermatozoa substances distribution and morphological differences between Holstein and Korean native cattle using three-dimensional refractive index …

H Jiang, J Kwon, S Lee, YJ Jo, S Namgoong, X Yao… - Scientific reports, 2019 - nature.com
is defined as the integration of the refractive index (RI) along an optical path; the topographic
information (optical thickness) of the sample is … stain-free interferometric phase microscopy

Simultaneous morphology, motility, and fragmentation analysis of live individual sperm cells for male fertility evaluation

K Ben-Yehuda, SK Mirsky, M Levi… - Advanced Intelligent …, 2022 - Wiley Online Library
… for sperm cells, such as Zernike phase contrast microscopy, differential interference contrast
(DIC) microscopy, and … (topographic) and content (refractive-index)-related values on all the …

High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa

M Rubessa, JM Feugang, ME Kandel… - Animal reproduction …, 2020 - Elsevier
Spatial light interference microscopy (SLIM) is an alternative microscopy system that allows
… of the nanoscale features of sperm morphometry and topography, while the Height Sensor …

Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos

MB Wheeler, RAC Rabel, M Rubessa… - Biology of …, 2024 - academic.oup.com
… QPI are spatial light interference microscopy (SLIM) [29] and gradient light interference
microscopy (GLIM) [30] microscopy. … of topography and refractometry of bovine spermatozoa [29] …

… : Application of optical nanoscopy, artificial intelligence-assisted quantitative phase microscopy and mitochondrial DNA copy numbers to assess human sperm cells

D Popova - 2021 - munin.uit.no
sperm cells and semen quality, which seems to be a relevant sperm parameter for routine
clinical practice in male fertility … about information of the refractive index and the cell’s local …

High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition

A Butola, D Popova, DK Prasad, A Ahmad, A Habib… - Scientific reports, 2020 - nature.com
… of the refractive index and the local thickness of the sperm cells. This quantitative morphological
information of the sperm cells is not obtained using a bright field microscopy. Additionally…