Toward quantitative metabarcoding

AO Shelton, ZJ Gold, AJ Jensen, ED′ Agnese… - Ecology, 2023 - Wiley Online Library
quantitative way, but many applications require quantitative information about the taxa or
systems under scrutiny. As metabarcoding … to improve the use of metabarcoding data in a wide …

How quantitative is metabarcoding: A meta‐analytical approach

PD Lamb, E Hunter, JK Pinnegar, S Creer… - Molecular …, 2019 - Wiley Online Library
… to which metabarcoding for taxonomic assignment is quantitative. Subsequently, a …
metabarcoding is quantitative across multiple independent studies. Factors affecting the quantitative

[PDF][PDF]  Towards quantitative metabarcoding of eukaryotic plankton: an approach to improve 18S rRNA gene copy number bias

JL Martin, I Santi, P Pitta, U John… - Metabarcoding and …, 2022 - mbmg.pensoft.net
… the inherent issue of quantitative metabarcoding for the entire … We therefore compared
the quantitative discrepancy … biomass relative abundances from metabarcoding reads if we …

Towards quantitative DNA Metabarcoding: A method to overcome PCR amplification bias

S Moinard, D Piau, F Laporte, D Rioux, P Taberlet… - bioRxiv, 2023 - biorxiv.org
… PCR Bias in metabarcoding Towards quantitative DNA Metabarcoding: A 1 method to …
and modeled in a similar way to fully achieve 554 the goal of truly quantitative metabarcoding. …

Metabarcoding as a quantitative tool for estimating biodiversity and relative biomass of marine zooplankton

EA Ershova, OS Wangensteen… - ICES Journal of …, 2021 - academic.oup.com
… an enhanced quantitative approach by metabarcoding whole … species richness using the
metabarcoding approach was 3–4 … were also recovered in the metabarcoding run. Within the …

Quantitative and qualitative assessment of pollen DNA metabarcoding using constructed species mixtures

KL Bell, KS Burgess, JC Botsch, EK Dobbs… - Molecular …, 2019 - Wiley Online Library
metabarcoding (… quantitative matching) is unclear, as these have not been assessed relative
to known standards. We tested the quantitative and qualitative robustness of metabarcoding

Quantification of marine benthic communities with metabarcoding

L Klunder, JDL van Bleijswijk… - Molecular Ecology …, 2022 - Wiley Online Library
… a insight in the quantitative abilities of DNA metabarcoding methods. We compared quantitative
… We showed that both approaches are limited in their quantitative abilities towards this …

Optimized metabarcoding with Pacific biosciences enables semi‐quantitative analysis of fungal communities

C Castaño, A Berlin, M Brandström Durling… - New …, 2020 - Wiley Online Library
Recent studies have questioned the use of high‐throughput sequencing of the nuclear
ribosomal internal transcribed spacer (ITS) region to derive a semi‐quantitative representation of …

[PDF][PDF] Does phylogeny explain bias in quantitative DNA metabarcoding?

M Liu, CP Burridge, LJ Clarke, SC Baker… - Metabarcoding and …, 2023 - mbmg.pensoft.net
… The causes of quantitative biases in metabarcoding are diverse and … on quantitative
metabarcoding should navigate the trade-offs of using alternative DNA markers. The quantitative

[HTML][HTML] Ecological indicators based on quantitative eDNA metabarcoding: the case of marine reserves

L Sanchez, E Boulanger, V Arnal, P Boissery… - Ecological …, 2022 - Elsevier
… New genetic techniques such as environmental DNA (eDNA) metabarcoding have emerged
and … In this paper, we propose a combination of metabarcoding and quantitative polymerase …