… complex, we have used electron cryotomography (cryo-ET) and … structural and cell biology
investigations inside bacterial cells (… other bacterial species remains largely uncharacterized. …

… , others are so far annotated as hypothetical or uncharacterized proteins. An alignment of
these proteins revealed conserved regions throughout the whole sequence, but especially at …

… Starting with a previously uncharacterized sample, a microscope operator typically screens
several grid squares with varying ice thicknesses then selects, by eye, the optimal squares …

… Several newly developed tilt schemes incorporating beam-tilt induced image shift
methods such as beam image-shift electron cryo-tomography (BISECT), parallel cryo-electron …

… Structural predictions of uncharacterized proteins identified in abundance in our sample
through mass spectrometry were also obtained with AlphaFold2. One protein, the product of …

… 7.12 The first structure of more than two domains of any MetH construct reveals a resting …
286 Figure 7.13 Individual domain conformations are consistent with previous MetH structures …

… This technique can contribute to understand cells at a structural and ultrastructural level
with both cryo- and room-temperature electron tomography. Furthermore, fluorescently labeled …

… Hitherto, the nanoscale organization of these networks has remained largely uncharacterized.
Cryo-electron tomography (cryo-ET) allowed to visualize and analyze the in situ …

Cryo-transmission electron microscopy (cryo-TEM) provides access to high resolution
information of adherent cell ultrastructures in a close to native environment but only volumes of …

… Some of them have been characterized, although it is highly likely that many new features
are present in uncharacterized bacteria. Bacteria that live in viscous environments need to …