Endocytosed nanogold fiducials for improved in-situ cryo–electron tomography tilt-series alignment

C Berger, RBG Ravelli, C López-Iglesias… - Journal of Structural …, 2021 - Elsevier
… This technique can contribute to understand cells at a structural and ultrastructural level
with both cryo- and room-temperature electron tomography. Furthermore, fluorescently labeled …

Conformational Control of Reactivity in Highly Flexible Enzymes Studied by Saxs and Cryo-Em

MB Watkins - 2022 - search.proquest.com
… 7.12 The first structure of more than two domains of any MetH construct reveals a resting …
286 Figure 7.13 Individual domain conformations are consistent with previous MetH structures

A novel widespread bacterial structure related to the flagellar type III secretion system

M Kaplan, CM Oikonomou, CR Wood, G Chreifi… - bioRxiv, 2021 - biorxiv.org
… While using electron cryo-tomography (cryo-ET) to study the process of flagellar assembly
in Helicobacter pylori, we identified a periplasmic hat-shaped structure embedded in the inner …

Archaeal type six secretion system mediates contact-dependent antagonism

T Zachs, JJL Malit, J Xu, A Schürch, S Sivabalasarma… - bioRxiv, 2024 - biorxiv.org
… Here we report the structure and function of haloarchaeal contractile injection systems (…
-electron tomography revealed CISs bound to the cytoplasmic membrane, resembling bacterial

Charting the molecular landscape of the cell

H Ochner, TAM Bharat - Structure, 2023 - cell.com
… One structural biology technique that has already been used to directly derive atomic … without
any additional external information, is electron cryotomography (cryoET). In this perspective …

Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus

M Kaplan, YW Chang, CM Oikonomou, WJ Nicolas… - bioRxiv, 2022 - biorxiv.org
… Bdellovibrio bacteriovorus is an endobiotic microbial predator that offers promise as a living
antibiotic for its ability to kill Gram-negative bacteria, including human pathogens. Even after …

Bacterial outer membrane polysaccharide export (OPX) proteins occupy three structural classes with selective β-barrel porin requirements for polymer secretion

F Saïdi, U Mahanta, A Panda, AA Kezzo… - Microbiology …, 2022 - Am Soc Microbiol
… of these 28 hits also revealed many with strong similarity to DUF6029, ascribing a potential
polysaccharide secretion role to this heretofore uncharacterized protein domain. MXAN_7418…

Micropatterning of cells on EM grids for efficient cryo-correlative light electron microscopy

L Swistak, A Sartori-Rupp, M Vos, J Enninga - Methods in Microbiology, 2021 - Elsevier
Cryo-transmission electron microscopy (cryo-TEM) provides access to high resolution
information of adherent cell ultrastructures in a close to native environment but only volumes of …

Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain

A Burt, CK Cassidy, P Ames, M Bacia-Verloop… - Nature …, 2020 - nature.com
… 5) of a complete transmembrane CSU provides several additional important structural
insights. First, the model allows us to describe the previously uncharacterized periplasmic …

Three-dimensional cilia structures from animals' closest unicellular relatives, the Choanoflagellates

JM Pinskey, A Lagisetty, L Gui, N Phan, E Reetz, G Fu… - bioRxiv, 2022 - biorxiv.org
… Indeed, the barb structures most closely resemble the size, shape, and general … an
uncharacterized bacterial complex in Prosthecobacter debontii, though the bacterial structures