Pseudouridine-free Ribosome Exhibits Distinct Inter-subunit Movements

Y Zhao, J Rai, H Yu, H Li - bioRxiv, 2021 - biorxiv.org
… The structural basis for how pseudouridine impacts ribosome function remains
uncharacterized. Here we report electron cryomicroscopy structures of a fully modified and a …

Atomic structures of an entire contractile injection system in both the extended and contracted states

A Desfosses, H Venugopal, T Joshi, J Felix… - Nature …, 2019 - nature.com
… Furthermore, we provide the structure of the AFP sheath–baseplate complex in a contracted
state. This study reveals atomic details of interaction networks that accompany and define …

Extracellular transfer of a conserved polymerization factor for multi-flagellin filament assembly in Caulobacter

N Kint, PH Viollier - Cell Reports, 2023 - cell.com
… Unidirectional growth of filamentous protein assemblies including the bacterial flagellum
relies on dedicated polymerization factors (PFs). The molecular determinants and structural

[图书][B] Gram-positive pathogens

VA Fischetti, RP Novick, JJ Ferretti, DA Portnoy… - 2019 - books.google.com
… (136) conducted studies of uncharacterized Gram-negative bacterial structures. These
structures will certainly open up a new avenue for research. The downside of the implementation …

Permafrost as a potential pathogen reservoir

R Wu, G Trubl, N Taş, JK Jansson - One Earth, 2022 - cell.com
… Permafrost is a reservoir of mostly uncharacterized microorganisms and viruses, many of …
as a microbial habitat and discuss what is known about the potential for microbial pathogens to …

Structural insights into the main S-layer unit of Deinococcus radiodurans reveal a massive protein complex with porin-like features

D Farci, MA Aksoyoglu, SF Farci, JA Bafna… - Journal of Biological …, 2020 - ASBMB
… As observed by low-resolution single-particle analysis, we show that the complex possesses
a porin-like structural organization, but is larger than other known porins. We also noted …

Polymorphic toxins and their immunity proteins: diversity, evolution, and mechanisms of delivery

ZC Ruhe, DA Low, CS Hayes - Annual review of microbiology, 2020 - annualreviews.org
Electron cryotomography reveals that this chamber contains a mass consistent with VgrG-tethered
effectors (29). Recent structural … cytoplasm through an uncharacterized mechanism. …

Biological Electron Cryotomography–Progress and Potential as Illustrated by the Dot/Icm Type IV Secretion System

G Jensen - 2023 - academic.oup.com
Recently electron cryotomography (cryo-ET) has made it possible to visualize large macromolecular
nanomachines inside intact cells in a near-native," frozen-hydrated" state in 3-D to a …

[HTML][HTML] Structural biology in cellulo: Minding the gap between conceptualization and realization

FL Kyrilis, JKK Low, JP Mackay, PL Kastritis - Current Opinion in Structural …, 2024 - Elsevier
… resolution snapshot of a cell is to use electron cryo-tomography (cryoET). In cryoET, micrographs
are … Bottom bar illustrates the complexity of cell extracts with uncharacterized structural

Positioning the model bacterial organelle, the carboxysome

JS MacCready, AG Vecchiarelli - MBio, 2021 - Am Soc Microbiol
… McdB-like proteins are found in other BMC-containing bacteria (41, 44) yet remain a novel
and uncharacterized family of proteins. We hypothesize that McdA gradients on the nucleoid …