… Thus, this study aimed to evaluate the diagnostic … TaqMan fluorogenic-labeled probes that
exploit the 5′ nuclease … Pearson correlation coefficient (r) was used in this study to assess …

… By contrast, the TaqMan assay, also known as fluorogenic 5′ nuclease assay, quantifies
through the addition of a probe labeled with fluorescent molecules that emit fluorescence …

… A 5' nuclease PCR assay for detection of the Y. pestis has … -PCR technique targeting the
caf1 gene was evaluated for … and Eisen RJ: Pentaplex real-time PCR for differential detection of …

… methods based on the polymerase chain reaction due to the … and in the system, which uses
MGB probes in multiplex mode [… using fluorogenic probes and the 5’ nuclease assay. Genetic …

… real-time PCR test combines 5′ nuclease technology with differentially labeled fluorogenic
… Five microliters of the template were used for each of the five multiplex PCR assays. An …

… nested polymerase chain reaction (nPCR) and a real-time PCR … nPCR and the real-time PCR
evaluated using plasmid DNA … the master mix PCR to validate their use on cheese samples …

… , including polymerase chain reaction-based and non-PCR-… Real-time PCR technology
creates an opportunity to monitor … by the 5′-nuclease activity of Taq-DNA polymerase as this …

… , and colorimetric polymerase chain reaction–based biosensor, as … Direct recognition
sensors are those that measure biological … To obtain multiple parameters in real-time at multiple …

… testing applications employ a method known as real-time … For the detection of food samples
5’ nuclease multiplex PCR … Detection of specific polymerase chain reaction product by …

… testing, such as DNA melting curve analysis, is required to confirm the generation of a specific
amplicon. The use … To evaluate the growth difference of L. monocytogenes in mushrooms …