… For quantification of red and green fluorescence and phenotypic … putida, we focused on
engineering a conditionally-… Elimination of the Ben catabolic activities of P. putida through …

… changes in the activation or repression of transcriptional activity by the P esaS and P esaR
… expression of the synthetic P esaS →trpD trpC cassette fully recovered the growth phenotype …

… studies to simple metabolic engineering manipulations), the … phosphate decarboxylase, an
essential activity for growth of P. … and thereby results in a filamentous phenotype), P. putida KT·…

… levels in the absence of phenotypic selection. Should desired … XylS/Pm expression system
and a customizable cassette … tion of activity of catabolic promoters of Pseudomonas putida …

… studies to simple metabolic engineering manipulations), the … deployment of complex prokaryotic
phenotypes. Nucleic Acids … As expected, the activity of nCas9 with spacers M1-2, and M1…

… enzyme engineering thus far aimed at improving catalytic efficiency, increasing activity and
… Four variants of an FRS expression cassette were constructed where msfGFP, encoding the …

… regulatory networks with intended phenotypic outputs. … In addition, alkS expression is subject
in Pseudomonas to … regulated expression systems under study, with basal activities of 0.075…

… and RsmZ, which antagonize the activity of the regulator RNA-… T3SS, we examined the
promoter activities of gacS, gacA, … many virulence-associated phenotypes, including swimming, …

… linear DNA-mediated genome engineering, often referred to as … the cassettes granted their
host a dark purple colony phenotype. On … The transposase activity is used for the copy number …

… the synthetic pathway of salicylic acid held phenotype for over 80 generations. … from
Pseudomonas putida MET94 was screened from random mutagenesis that exhibited optimal activity …