… for gene function studies in dicotyledonous plants, but far fewer are available for studies with
monocotyledonous plants [… is more amenable to study gene functions associated with plant …

… study gene functions without using stable transformation protocols represent RNAi and
virus-induced gene silencing … of the plants is more effective with regard to gene silencing and …

… Tobacco rattle virus-VIGS has also been used to study the functional roles of disease
resistance genes in soybean. Through the use of VIGS assays in soybean, Liu et al. (2015) …

… gene functions that is accomplished by mutagenesis. For a long time, genome editing in
flowering plants was a random process … briefly review high-impact VIGS-based studies from the …

… of this method to silence the QA biosynthetic gene LaLDC to provide direct evidence of
its role in QA … Therefore, we suggest inoculating at least 50 plants per construct for co-silencing. …

… the genes in the studied plants that are not usually accessible to transgenic techniques. We
… RNAs with specialized roles in gene silencing [11]. These RNAs have been used to identify …

… In the study of identifying gene function in plants, it is mainly … tool to identify gene functions
due to its rapid, efficient and high-… In this study, two infection methods (direct injection of leaves …

… (VIGS) can perform large-scale and rapid gene silencing in plants due to a reduction in the
… In this regard, we can use VIGS technology to study more gene functions [21]. Currently, in …

… functions of the genes you will study in the plant you are working on. With this issue of Methods
… /or optimize their own silencing experiments according to the methods described herein. …

… of virus induced gene silencing to improve plants. At first, a … RNAs and role of RNA silencing
is provided, with a role on the … for the study of functional genes in some types of plants such …