… in living cells — is one of the most recent advances in the field of genome … cells process
base editing intermediates may additionally inform new therapeutic strategies using base editors…

… Live cell images were acquired using a Nikon Eclipse Ti inverted microscope. Image
acquisition was performed at room temperature and air in a 24-well plate. Sickled …

… Adenine base editors (ABEs) convert targeted A•T base pairs to G•C in living cells without
requiring DSBs or donor DNA templates, and with minimal formation of indels 2 . In SCD, the …

… Because new base editors significantly reduce unintended editing in the … base editors,
assess their technical advantages and limitations, and discuss their broad applications in basic …

… Base editing Base editing is a new technology for the precise modification of genomes (DNA)
or transcriptomes (RNA) of living cells at the single-base resolution. BEs are composed of …

… of point mutations in living cells, we developed base editors—… deliver base editors using
AAVs, we devised a split base-editor … Each base-editor half is fused to half of a fast-splicing split …

… off-target RNA-editing activity of DNA base editors, expands the options available to minimize
these unwanted effects, and provides new SECURE base editor architectures with other …

… do not fully capture the scope of base editing. A systematic and comprehensive … of base
editing would enhance our understanding of base editors, facilitate their use in precision editing …

… Although ALS is currently incurable, CRISPR base editors hold … in vivo delivery of cytidine
base editors (CBEs) consisting of the … This work expands the capabilities of single-base editors …

… In an effort to create adenine base editors enabling A-to-G substitutions in organelle DNA,
we first investigated whether fusion proteins containing a TALE protein custom designed to …