… methylation evaluation with single-base resolution. Despite the advantages of these
approaches, DNA methylation differences … DNA must be fully denatured (single stranded) to ease …

… The differences between these features form the basis of the DNA methylation analysis (…
In 1999, Aggerhoim developed a methylation-specific denaturing gradient gel electrophoresis (…

… our understanding of the methylation regulation. Genome-wide analyses such as microarrays
and … The methylation status of the control regions may differ between cell types, and thus …

… the measurement of cytosine methylation from specific loci at … In this review, we discuss the
different DNA methylation analysis … platforms for genome-wide analysis of DNA methylation. …

… The most critical step in bisulfite analysis of DNA methylation … ensuring efficient DNA
denaturation and subsequent sulfonation … the maximal Tm difference between the “methylated” …

… conditions for all of the reactions were as follows: denaturation at 95C for 15 s, annealing at
60C … The temperature differences between paternal and maternal allele are due to the CpG …

… A key difference between antibody-based and MBD protein-… Hence, for MeDIP denatured
DNA has to be used, whereas MBD … development differences in the localization of methylated …

… perform the real-time PCR analysis. Each reaction started with a denaturation step at 95C for
… To shed light on the observed difference in global demethylation but not methylation levels, …

… The amplified segments are subsequently denatured and run on a nondenaturing
polyacrylamide gel. Strands that differ by as little as a single base substitution can form different …

… The choice of cloning approach can make a huge difference in the whole process. Since a
single … Antibodies are better for denatured ssDNA with a low density of CpGs while MBDs bind …