Antibody orientational labeling via staphylococcus A protein to improve the sensitivity of gold immunochromatography assays
P Zhao, X Huang, H Tao, Y Li, L Sun, J Hu - Analytical Biochemistry, 2022 - Elsevier
… In the present study, a simple and effective mark method of antibody to colloidal gold
nanoparticles was developed and evaluated. The rationale of immobilization of Antibody onto …
nanoparticles was developed and evaluated. The rationale of immobilization of Antibody onto …
Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays: A review
… the assays and metal nanoparticles are found to be significantly advantageous for improving
the sensitivity of these assays [… Immunogold labeling technique has also been employed to …
the sensitivity of these assays [… Immunogold labeling technique has also been employed to …
Direct quantification of surface coverage of antibody in IgG-Gold nanoparticles conjugates
L Zhang, D Hu, M Salmain, B Liedberg, S Boujday - Talanta, 2019 - Elsevier
… protein assay [21]. However, despite careful calibration treatment, ie removal of nanoparticles
… the interference from nanoparticles, dissociation of antibody from nanoparticles prior to …
… the interference from nanoparticles, dissociation of antibody from nanoparticles prior to …
Double amplification upon immuno-gold nanoparticles promoted trace measurement of ricin by biolayer interferometry
L Luo, H Xu, C Wang, Z Li, L Guo, X Li, L Luo… - Sensors and Actuators B …, 2022 - Elsevier
… In this article, we contributed a double amplification strategy upon immuno-gold
nanoparticles (immunoGNPs) in BLI, which promoted a measurement of trace ricin based on the …
nanoparticles (immunoGNPs) in BLI, which promoted a measurement of trace ricin based on the …
Optimization and structural stability of gold nanoparticle–antibody bioconjugates
… Additional UV–vis measurements were used to determine the concentration of unbounded
antibody remaining in the supernatant via the BCA protein assay method. This equation is …
antibody remaining in the supernatant via the BCA protein assay method. This equation is …
Interaction between gold nanoparticles and blood proteins to define disease states
DJ Peitinho, MP de Almeida, E Pereira… - Annals of …, 2019 - Taylor & Francis
… , and characterized for GRP content through TEM-immunogold staining, Western blot, ELISA,
qPCR. Functional assays using isolated EVs containing GRP were performed in primary …
qPCR. Functional assays using isolated EVs containing GRP were performed in primary …
Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
M Tvrdonova, M Vlcnovska, LP Vanickova… - Analytical and …, 2019 - Springer
… In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with
diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass …
diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass …
[HTML][HTML] Development of a rapid gold nanoparticle-based lateral flow immunoassay for the detection of dengue virus
C Martinez-Liu, C Machain-Williams, N Martinez-Acuña… - Biosensors, 2022 - mdpi.com
… based on gold nanoparticles (AuNPs) and antibody 4G2 for the detection of dengue virus (…
assay (LFA) for the development of an alternative test to detect the flavivirus envelope protein …
assay (LFA) for the development of an alternative test to detect the flavivirus envelope protein …
Antibody-gold nanoparticle bioconjugates for biosensors: synthesis, characterization and selected applications
L Zhang, Y Mazouzi, M Salmain, B Liedberg… - Biosensors and …, 2020 - Elsevier
… This additional step, leading to the presence of another protein, makes the previous classical
protein assays inapplicable. In this case, antibody assay in the supernatant still can be …
protein assays inapplicable. In this case, antibody assay in the supernatant still can be …
Development of a rapid dot-blot assay for ochratoxin A (OTA) detection using peptide conjugated gold nanoparticles for bio-recognition and detection
… In this study, a rapid, user-friendly dot-blot assay was developed using peptide conjugated
gold nanoparticles (AuNPs) as a detection agent. The peptide binding to ochratoxin A was …
gold nanoparticles (AuNPs) as a detection agent. The peptide binding to ochratoxin A was …
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