Antibody orientational labeling via staphylococcus A protein to improve the sensitivity of gold immunochromatography assays

P Zhao, X Huang, H Tao, Y Li, L Sun, J Hu - Analytical Biochemistry, 2022 - Elsevier
… In the present study, a simple and effective mark method of antibody to colloidal gold
nanoparticles was developed and evaluated. The rationale of immobilization of Antibody onto …

Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays: A review

MS Tabatabaei, R Islam, M Ahmed - Analytica Chimica Acta, 2021 - Elsevier
… the assays and metal nanoparticles are found to be significantly advantageous for improving
the sensitivity of these assays [… Immunogold labeling technique has also been employed to …

Direct quantification of surface coverage of antibody in IgG-Gold nanoparticles conjugates

L Zhang, D Hu, M Salmain, B Liedberg, S Boujday - Talanta, 2019 - Elsevier
protein assay [21]. However, despite careful calibration treatment, ie removal of nanoparticles
… the interference from nanoparticles, dissociation of antibody from nanoparticles prior to …

Double amplification upon immuno-gold nanoparticles promoted trace measurement of ricin by biolayer interferometry

L Luo, H Xu, C Wang, Z Li, L Guo, X Li, L Luo… - Sensors and Actuators B …, 2022 - Elsevier
… In this article, we contributed a double amplification strategy upon immuno-gold
nanoparticles (immunoGNPs) in BLI, which promoted a measurement of trace ricin based on the …

Optimization and structural stability of gold nanoparticle–antibody bioconjugates

RT Busch, F Karim, J Weis, Y Sun, C Zhao… - ACS …, 2019 - ACS Publications
… Additional UV–vis measurements were used to determine the concentration of unbounded
antibody remaining in the supernatant via the BCA protein assay method. This equation is …

Interaction between gold nanoparticles and blood proteins to define disease states

DJ Peitinho, MP de Almeida, E Pereira… - Annals of …, 2019 - Taylor & Francis
… , and characterized for GRP content through TEM-immunogold staining, Western blot, ELISA,
qPCR. Functional assays using isolated EVs containing GRP were performed in primary …

Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

M Tvrdonova, M Vlcnovska, LP Vanickova… - Analytical and …, 2019 - Springer
… In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with
diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass …

[HTML][HTML] Development of a rapid gold nanoparticle-based lateral flow immunoassay for the detection of dengue virus

C Martinez-Liu, C Machain-Williams, N Martinez-Acuña… - Biosensors, 2022 - mdpi.com
… based on gold nanoparticles (AuNPs) and antibody 4G2 for the detection of dengue virus (…
assay (LFA) for the development of an alternative test to detect the flavivirus envelope protein

Antibody-gold nanoparticle bioconjugates for biosensors: synthesis, characterization and selected applications

L Zhang, Y Mazouzi, M Salmain, B Liedberg… - Biosensors and …, 2020 - Elsevier
… This additional step, leading to the presence of another protein, makes the previous classical
protein assays inapplicable. In this case, antibody assay in the supernatant still can be …

Development of a rapid dot-blot assay for ochratoxin A (OTA) detection using peptide conjugated gold nanoparticles for bio-recognition and detection

S Rahi, V Lanjekar, V Ghormade - Food control, 2022 - Elsevier
… In this study, a rapid, user-friendly dot-blot assay was developed using peptide conjugated
gold nanoparticles (AuNPs) as a detection agent. The peptide binding to ochratoxin A was …