Estimation of gene expression levels plays a crucial role in understanding the function of the
target gene (s). Intersample variance in gene expression can be more precisely measured if …

Background Normalizing through reference genes, or housekeeping genes, can make more
accurate and reliable results from reverse transcription real-time quantitative polymerase …

Background Cotton is one of the most important commercial crops as the source of natural
fiber, oil and fodder. To protect it from harmful pest populations number of newer transgenic …

Objective To identify reliable reference genes for gene expression analysis in Gossypium
raimondii. Results Five different software tools, geNorm, NormFinder, BestKeeper, ReFinder …

Cotton (Gossypium hirsutum) is the most important non-food plant in the world. Studies
concerning the fiber quality and plant fitness of cotton at molecular level depend on high …

Plants reshape their transcriptomes, proteomes and metabolomes in response to insect
damage. In this study, we used suppression subtractive hybridization to investigate the …

Quantitative Real-Time PCR (qPCR) is a preferred and reliable method for accurate
quantification of gene expression to understand precise gene functions. A total of 25 …

Cotton production in Pakistan is vital for economic development of the country. It contributes
around 0.6 percent to GDP and 3.1 percent of the value added in agriculture for the year …

Adzuki bean (Vigna angularis) is one of the most important legume crops in Asian countries
like China, Japan and Korea due to its nutritious protein and starch contents. In spite of its …

Quantitative real-time polymerase chain reaction (qRT-PCR) is a sensitive and widely used
technique for quantifying gene expression levels, and its accuracy depends on the reference …