Long-term effect on in vitro cloning efficiency after treatment of somatic cells with Xenopus egg extract in the pig

Y Liu, O Østrup, R Li, J Li, G Vajta… - Reproduction …, 2014 - CSIRO Publishing
In somatic cell nuclear transfer (SCNT), donor cell reprogramming is considered as a
biologically important and vulnerable event. Various donor cell pre-treatments with Xenopus …

Improved in vitro development of cloned bovine embryos using S‐adenosylhomocysteine, a non‐toxic epigenetic modifying reagent

S Jafari, MS Hosseini, M Hajian… - Molecular …, 2011 - Wiley Online Library
In this study, fibroblast cells were stably transfected with mouse POU5F1 promoter‐driven
enhanced green fluorescent protein (EGFP) to investigate the effect of S …

[HTML][HTML] Inhibition of aberrant DNA re-methylation improves post-implantation development of somatic cell nuclear transfer embryos

R Gao, C Wang, Y Gao, W Xiu, J Chen, X Kou, Y Zhao… - Cell stem cell, 2018 - cell.com
Somatic cell nuclear transfer (SCNT) enables cloning of differentiated cells by
reprogramming their nuclei to a totipotent state. However, successful full-term development …

[HTML][HTML] DNA methylation patterns in tissues from mid-gestation bovine foetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear …

C Couldrey, RSF Lee - BMC developmental biology, 2010 - Springer
Background Cloning of cattle by somatic cell nuclear transfer (SCNT) is associated with a
high incidence of pregnancy failure characterized by abnormal placental and foetal …

[HTML][HTML] Genome-wide dynamic profiling of histone methylation during nuclear transfer-mediated porcine somatic cell reprogramming

Z Cao, Y Li, Z Chen, H Wang, M Zhang, N Zhou, R Wu… - PloS one, 2015 - journals.plos.org
The low full-term developmental efficiency of porcine somatic cell nuclear transfer (SCNT)
embryos is mainly attributed to imperfect epigenetic reprogramming in the early embryos …

Epigenetic reprogramming, gene expression and in vitro development of porcine SCNT embryos are significantly improved by a histone deacetylase inhibitor—m …

Y Song, T Hai, Y Wang, R Guo, W Li, L Wang… - Protein & …, 2014 - academic.oup.com
Insufficient epigenetic reprogramming of donor nuclei is believed to be one of the most
important causes of low development efficiency of mammalian somatic cell nuclear transfer …

[PDF][PDF] Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos

J Cho, S Kang, BC Lee - Journal of Veterinary Science, 2014 - synapse.koreamed.org
This study was conducted to investigate the expression of three genes related to early
embryonic development in bovine transgenic cloned embryos. To accomplish this …

Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos

KI Yamanaka, K Yamashita, H Khatun… - Animal Science …, 2018 - Wiley Online Library
Epigenetic reprogramming confers totipotency even during somatic cell nuclear transfer
(SCNT), which has been used to clone various animal species. However, as even …

[HTML][HTML] Simultaneous inhibition of histone deacetylases and RNA synthesis enables totipotency reprogramming in pig SCNT embryos

MP de Macedo, WG Glanzner, K Gutierrez… - International Journal of …, 2022 - mdpi.com
Combining somatic cell nuclear transfer (SCNT) with genome editing technologies has
emerged as a powerful platform for the creation of unique swine lineages for agricultural and …

Analysis of apoptosis and methyltransferase mRNA expression in porcine cloned embryos cultured in vitro

S Ju, R Rui, Q Lu, P Lin, H Guo - Journal of assisted reproduction and …, 2010 - Springer
Purpose The purpose of this study was to investigate the relationship of porcine somatic cell
nuclear transfer (SCNT) embryo developmental competence with embryonic cell apoptosis …