Raman imaging using molecular reporters is a relatively new approach in subcellular
investigations. It enables the visualization of organelles in cells with better selectivity and …

Endothelial cells (EC) constitute a single layer of the lining of blood vessels and play an
important role in maintaining cardiovascular homeostasis. Endothelial dysfunction has been …

Raman microscopy, a label-free method with high spatial resolution, shows growing
potential in various fields of medical diagnostics. Several proof-of-concept studies related to …

In this work, confocal Raman imaging was used to study the formation of lipid droplets (LDs)
in vitro in a single endothelial cell upon incubation with polyunsaturated fatty acids (10 or 25 …

Raman microspectroscopy is one of the most powerful tools in molecular sensing, offering a
non-invasive and comprehensive characterization of the intracellular environment. To …

Multiple diseases are at some point associated with altered endothelial function, and
endothelial dysfunction (ED) contributes to their pathophysiology. Biochemical changes of …

In this work, 3D linear Raman spectroscopy was used to study lipid droplets (LDs) ex vivo in
liver tissue and also in vitro in a single endothelial cell. Spectroscopic measurements …

Highlights•Raman microscopy with parallel spectral acquisition provides about two orders of
magnitude improvement in imaging speed.•High-speed Raman imaging captured various …

Raman spectroscopy is successfully becoming an analytical tool used to characterize
alterations in the biochemical composition of cells. In this work, we identify the features of …

Growing interest in the role of endothelium under physiological and pathological conditions
has led to an increasing demand for its representative in vitro models especially suitable for …