Dissertation Page 1 Structure of mammalian RNA polymerase II elongation complex bound by
α-amanitin and study of mammalian transcription termination and 3’ end processing Dissertation …

Our laboratory has developed methods for transient state kinetic analysis of human RNA
polymerase II elongation. In these studies, multiple conformations of the RNA polymerase II …

Eukaryotic RNA polymerase II is a complex enzyme composed of 12 distinct subunits that is
present in cells in low abundance. Transcription of mRNA by RNA polymerase II involves a …

Using defined elongation complexes formed on dC-tailed templates with Drosophila RNA
polymerase II, we have examined elongation, pyrophosphorolysis, and DmS-II-mediated …

Structural basis of transcription: α-Amanitin–RNA polymerase II cocrystal at 2.8 Å resolution |
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RNA polymerase II (Pol II) is the central enzyme that transcribes eukaryotic protein-coding
genes to produce mRNA. The mushroom toxin α-amanitin binds Pol II and inhibits …

The elongation phase of transcription by RNA polymerase II (RNAP II) is tightly controlled by
a large number of transcription elongation factors. Here we describe experimental …

RNA polymerase II is the eukaryotic enzyme that transcribes all the mRNA in the cell.
Complex mechanisms of transcription and its regulation underlie basic functions including …

Immobilized DNA templates, glycerol gradient centrifugation, and native gel analysis were
utilized to isolate and compare functional RNA polymerase II (RNAPII) elongation …

Eukaryotic DNA-dependent RNA polymerases are multimeric enzymes consisting of 1&15
polypeptides (Roeder, 1976). Information about the role of subunits in transcription of …