Re-sequencing and optical mapping reveals misassemblies and real inversions on Corynebacterium pseudotuberculosis genomes

TJ Sousa, D Parise, R Profeta, MTD Parise… - Scientific Reports, 2019 - nature.com
The number of draft genomes deposited in Genbank from the National Center for
Biotechnology Information (NCBI) is higher than the complete ones. Draft genomes are …

Whole-genome optical mapping reveals a mis-assembly between two rRNA operons of Corynebacterium pseudotuberculosis strain 1002

DCB Mariano, TJ Sousa, FL Pereira, F Aburjaile… - BMC genomics, 2016 - Springer
Background Studies have detected mis-assemblies in genomes of the species
Corynebacterium pseudotuberculosis. These new discover have been possible due to the …

[HTML][HTML] Rapid hybrid de novo assembly of a microbial genome using only short reads: Corynebacterium pseudotuberculosis I19 as a case study

LT Cerdeira, AR Carneiro, RTJ Ramos… - Journal of …, 2011 - Elsevier
Due to the advent of the so-called Next-Generation Sequencing (NGS) technologies the
amount of monetary and temporal resources for whole-genome sequencing has been …

A case study into microbial genome assembly gap sequences and finishing strategies

SM Utturkar, DM Klingeman, RA Hurt Jr… - Frontiers in …, 2017 - frontiersin.org
This study characterized regions of DNA which remained unassembled by either PacBio
and Illumina sequencing technologies for seven bacterial genomes. Two genomes were …

An overlooked phenomenon: complex interactions of potential error sources on the quality of bacterial de novo genome assemblies

Z Rádai, A Váradi, P Takács, NA Nagy, N Schmitt… - BMC genomics, 2024 - Springer
Background Parameters adversely affecting the contiguity and accuracy of the assemblies
from Illumina next-generation sequencing (NGS) are well described. However, past studies …

High efficiency application of a mate-paired library from next-generation sequencing to postlight sequencing: Corynebacterium pseudotuberculosis as a case study for …

RTJ Ramos, AR Carneiro, S de Castro Soares… - Journal of …, 2013 - Elsevier
With the advent of high-throughput DNA sequencing platforms, there has been a reduction
in the cost and time of sequencing. With these advantages, new challenges have emerged …

[HTML][HTML] Simplifier: a web tool to eliminate redundant NGS contigs

RTJ Ramos, AR Carneiro, V Azevedo… - …, 2012 - ncbi.nlm.nih.gov
Modern genomic sequencing technologies produce a large amount of data with reduced
cost per base; however, this data consists of short reads. This reduction in the size of the …

Tips and tricks for the assembly of a Corynebacterium pseudotuberculosis genome using a semiconductor sequencer

RTJ Ramos, AR Carneiro, SC Soares… - Microbial …, 2013 - Wiley Online Library
New sequencing platforms have enabled rapid decoding of complete prokaryotic genomes
at relatively low cost. The I on T orrent platform is an example of these technologies …

GFinisher: a new strategy to refine and finish bacterial genome assemblies

D Guizelini, RT Raittz, LM Cruz, EM Souza… - Scientific reports, 2016 - nature.com
Despite the development in DNA sequencing technology, improving the number and the
length of reads, the process of reconstruction of complete genome sequences, the so called …

Hound: a novel tool for automated mapping of genotype to phenotype in bacterial genomes assembled de novo

C Reding, N Satapoomin… - Briefings in …, 2024 - academic.oup.com
Increasing evidence suggests that microbial species have a strong within species genetic
heterogeneity. This can be problematic for the analysis of prokaryote genomes, which …