Background It remains a challenge to perform de novo assembly using next-generation
sequencing (NGS). Despite the availability of multiple sequencing technologies and tools …

Background Next generation sequencing technology has allowed efficient production of
draft genomes for many organisms of interest. However, most draft genomes are just …

Motivation: To assess the potential of different types of sequence data combined with de
novo and hybrid assembly approaches to improve existing draft genome sequences …

Genome assembly has always been complicated due to the inherent difficulties of
sequencing technologies, as well the computational methods used to process sequences …

The reconstruction of the complete genome sequence of an organism is an important point
for comparative, functional and evolutionary genomics. Nevertheless, overcoming the …

Contamination in sequenced genomes is a relatively common problem and several methods
to remove non-target sequences have been devised. Typically, the target and contaminating …

Recent developments in sequencing technologies have given the opportunity to sequence
many bacterial genomes with limited cost and labor, compared to previous techniques …

Background With the rapid development of the next generation sequencing (NGS)
technology, large quantities of genome sequencing data have been generated. Because of …

Genome assembly uses sequence similarity to go from sequencing reads to longer
contiguous sequences (contigs). Scaffolds are contigs linked together by gaps where the …

A wide variety of genome sequencing platforms have emerged in the recent past. High-
throughput platforms like Illumina and 454 are essentially adaptations of the shotgun …