High-throughput functional variant screens via in vivo production of single-stranded DNA
MG Schubert, DB Goodman… - Proceedings of the …, 2021 - National Acad Sciences
Creating and characterizing individual genetic variants remains limited in scale, compared
to the tremendous variation both existing in nature and envisioned by genome engineers …
to the tremendous variation both existing in nature and envisioned by genome engineers …
CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window
The capacity to diversify genetic codes advances our ability to understand and engineer
biological systems,. A method for continuously diversifying user-defined regions of a …
biological systems,. A method for continuously diversifying user-defined regions of a …
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
AD Garst, MC Bassalo, G Pines, SA Lynch… - Nature …, 2017 - nature.com
Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
Comparative optimization of combinatorial CRISPR screens
Combinatorial CRISPR technologies have emerged as a transformative approach to
systematically probe genetic interactions and dependencies of redundant gene pairs …
systematically probe genetic interactions and dependencies of redundant gene pairs …
High-throughput retrieval of target sequences from complex clone libraries using CRISPRi
J Burian, VK Libis, YA Hernandez… - Nature …, 2023 - nature.com
The capture of metagenomic DNA in large clone libraries provides the opportunity to study
microbial diversity that is inaccessible using culture-dependent methods. In this study, we …
microbial diversity that is inaccessible using culture-dependent methods. In this study, we …
Genome-scale CRISPR pooled screens
NE Sanjana - Analytical biochemistry, 2017 - Elsevier
Genome editing technologies such as clustered regularly interspaced short palindromic
repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The …
repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The …
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
ASL Wong, GCG Choi, CH Cui… - Proceedings of the …, 2016 - National Acad Sciences
The orchestrated action of genes controls complex biological phenotypes, yet the systematic
discovery of gene and drug combinations that modulate these phenotypes in human cells is …
discovery of gene and drug combinations that modulate these phenotypes in human cells is …
CRISPR Visualizer: rapid identification and visualization of CRISPR loci via an automated high-throughput processing pipeline
MA Nethery, R Barrangou - RNA biology, 2019 - Taylor & Francis
ABSTRACT A CRISPR locus, defined by an array of repeat and spacer elements, constitutes
a genetic record of the ceaseless battle between bacteria and viruses, showcasing the …
a genetic record of the ceaseless battle between bacteria and viruses, showcasing the …
Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi
The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …
genetic tools needed to systematically associate genes with phenotypes. This is the major …
Next-generation forward genetic screens: uniting high-throughput perturbations with single-cell analysis
JA Morris, JS Sun, NE Sanjana - Trends in Genetics, 2023 - cell.com
Programmable genome-engineering technologies, such as CRISPR (clustered regularly
interspaced short palindromic repeats) nucleases and massively parallel CRISPR screens …
interspaced short palindromic repeats) nucleases and massively parallel CRISPR screens …