Creating and characterizing individual genetic variants remains limited in scale, compared
to the tremendous variation both existing in nature and envisioned by genome engineers …

The capacity to diversify genetic codes advances our ability to understand and engineer
biological systems,. A method for continuously diversifying user-defined regions of a …

Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

Combinatorial CRISPR technologies have emerged as a transformative approach to
systematically probe genetic interactions and dependencies of redundant gene pairs …

The capture of metagenomic DNA in large clone libraries provides the opportunity to study
microbial diversity that is inaccessible using culture-dependent methods. In this study, we …

Genome editing technologies such as clustered regularly interspaced short palindromic
repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The …

The orchestrated action of genes controls complex biological phenotypes, yet the systematic
discovery of gene and drug combinations that modulate these phenotypes in human cells is …

ABSTRACT A CRISPR locus, defined by an array of repeat and spacer elements, constitutes
a genetic record of the ceaseless battle between bacteria and viruses, showcasing the …

The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …

Programmable genome-engineering technologies, such as CRISPR (clustered regularly
interspaced short palindromic repeats) nucleases and massively parallel CRISPR screens …