A localized multimeric anchor attaches the Caulobacter holdfast to the cell pole

GG Hardy, RC Allen, E Toh, M Long… - Molecular …, 2010 - Wiley Online Library
Caulobacter crescentus attachment is mediated by the holdfast, a complex of
polysaccharide anchored to the cell by HfaA, HfaB and HfaD. We show that all three proteins …

The HfaB and HfaD adhesion proteins of Caulobacter crescentus are localized in the stalk

JL Cole, GG Hardy, D Bodenmiller, E Toh… - Molecular …, 2003 - Wiley Online Library
The differentiating bacterium Caulobacter crescentus produces two different cell types at
each cell division, a motile swarmer cell and an adhesive stalked cell. The stalked cell …

The Caulobacter crescentus holdfast: Identification of holdfast attachment complex genes

HD Kurtz Jr, J Smith - FEMS microbiology letters, 1994 - academic.oup.com
Caulobacters are biofilm bacteria that attach to surfaces via a holdfast, an adhesive
expressed at discrete cell surface sites. We have described a cluster of at least three genes …

HfaE is a component of the holdfast anchor complex that tethers the holdfast adhesin to the cell envelope

NK Chepkwony, GG Hardy, YV Brun - Journal of Bacteriology, 2022 - Am Soc Microbiol
Bacteria use adhesins to colonize different surfaces and form biofilms. The species of the
Caulobacterales order use a polar adhesin called holdfast, composed of polysaccharides …

Analysis of a Caulobacter crescentus gene cluster involved in attachment of the holdfast to the cell

HD Kurtz Jr, J Smith - Journal of bacteriology, 1992 - Am Soc Microbiol
Caulobacter crescentus firmly adheres to surfaces with a structure known as the holdfast,
which is located at the flagellar pole of swarmer cells and at the stalk tip in stalked cells. A …

A multiprotein complex anchors adhesive holdfast at the outer membrane of Caulobacter crescentus

NI Sulkowski, GG Hardy, YV Brun… - Journal of …, 2019 - Am Soc Microbiol
Adhesion allows microbes to colonize surfaces and is the first stage in biofilm formation.
Stable attachment of the freshwater alphaproteobacterium Caulobacter crescentus to …

Identification of Genes Required for Synthesis of the Adhesive Holdfast in Caulobacter crescentus

CS Smith, A Hinz, D Bodenmiller, DE Larson, YV Brun - 2003 - Am Soc Microbiol
Adhesion to both abiotic and biotic surfaces by the gram-negative prothescate bacterium
Caulobacter crescentus is mediated by a polar organelle called the “holdfast,” which …

Identification of genes affecting production of the adhesion organelle of Caulobacter crescentus CB2

D Mitchell, J Smit - Journal of bacteriology, 1990 - Am Soc Microbiol
Transposon (Tn5) mutagenesis was used to identify regions in the genome involved with
production, regulation, or attachment to the cell surface of the adhesive holdfast of the …

Mutations in sugar-nucleotide synthesis genes restore holdfast polysaccharide anchoring to Caulobacter crescentus holdfast anchor mutants

GG Hardy, E Toh, C Berne, YV Brun - Journal of bacteriology, 2018 - Am Soc Microbiol
Attachment is essential for microorganisms to establish interactions with both biotic and
abiotic surfaces. Stable attachment of Caulobacter crescentus to surfaces requires an …

Attachment of the adhesive holdfast organelle to the cellular stalk of Caulobacter crescentus

CJ Ong, ML Wong, J Smit - Journal of bacteriology, 1990 - Am Soc Microbiol
Caulobacters attach to surfaces in the environment via their holdfasts, attachment organelles
located at the base of the flagellum in swarmer cells and later at the end of the cellular stalk …