Structure–function analysis of bacterial GH31 α‐galactosidases specific for α‐(1→ 4)‐galactobiose

M Ikegaya, EY Park, T Miyazaki - The FEBS Journal, 2023 - Wiley Online Library
Glycoside hydrolase family 31 (GH31) contains α‐glycoside hydrolases with different
substrate specificities involved in various physiological functions. This family has recently …

Structural and biochemical characterization of novel bacterial α-galactosidases belonging to glycoside hydrolase family 31

T Miyazaki, Y Ishizaki, M Ichikawa… - Biochemical …, 2015 - portlandpress.com
Glycoside hydrolase family 31 (GH31) proteins have been reportedly identified as exo-α-
glycosidases with activity for α-glucosides and α-xylosides. We focused on a GH31 …

[HTML][HTML] GH97 is a new family of glycoside hydrolases, which is related to the α-galactosidase superfamily

DG Naumoff - BMC genomics, 2005 - Springer
Background As a rule, about 1% of genes in a given genome encode glycoside hydrolases
and their homologues. On the basis of sequence similarity they have been grouped into …

A novel glycoside hydrolase family 97 enzyme: Bifunctional β-L-arabinopyranosidase/α-galactosidase from Bacteroides thetaiotaomicron

A Kikuchi, M Okuyama, K Kato, S Osaki, M Ma… - Biochimie, 2017 - Elsevier
Abstract Glycoside hydrolase family 97 (GH97) is one of the most interesting glycosidase
families, which contains inverting and retaining glycosidases. Currently, only two enzyme …

Crystal structure of the Enterococcus faecalis α‐N‐acetylgalactosaminidase, a member of the glycoside hydrolase family 31

T Miyazaki, EY Park - FEBS letters, 2020 - Wiley Online Library
Glycoside hydrolases catalyze the hydrolysis of glycosidic linkages in carbohydrates. The
glycoside hydrolase family 31 (GH31) contains α‐glucosidase, α‐xylosidase, α …

Unraveling the Substrate Recognition Mechanism and Specificity of the Unusual Glycosyl Hydrolase Family 29 BT2192 from Bacteroides thetaiotaomicron

L Guillotin, P Lafite, R Daniellou - Biochemistry, 2014 - ACS Publications
Glycosyl hydrolase (GH) family 29 (CAZy database) consists of retaining α-l-fucosidases. We
have identified BT2192, a protein from Bacteroides thetaiotaomicron, as the first GH29 …

[HTML][HTML] Structural basis of the strict specificity of a bacterial GH31 α-1, 3-glucosidase for nigerooligosaccharides

M Ikegaya, T Moriya, N Adachi, M Kawasaki… - Journal of Biological …, 2022 - ASBMB
Carbohydrate-active enzymes are involved in the degradation, biosynthesis, and
modification of carbohydrates and vary with the diversity of carbohydrates. The glycoside …

Crystal structure of α-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding

F Fredslund, M Abou Hachem, RJ Larsen… - Journal of molecular …, 2011 - Elsevier
Lactobacillus acidophilus NCFM is a probiotic bacterium known for its beneficial effects on
human health. The importance of α-galactosidases (α-Gals) for growth of probiotic …

PspAG97A: A halophilic α-glucoside hydrolase with wide substrate specificity from glycoside hydrolase family 97

W Li, H Fan, C He, X Zhang, X Wang… - … of Microbiology and …, 2016 - koreascience.kr
Abstract A novel ${\alpha}-glucoside $ hydrolase (named PspAG97A) from glycoside
hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas …

Biochemical Analysis of Thermotoga maritima GH36 α-Galactosidase (TmGalA) Confirms the Mechanistic Commonality of Clan GH-D Glycoside Hydrolases

DA Comfort, KS Bobrov, DR Ivanen, KA Shabalin… - Biochemistry, 2007 - ACS Publications
Organization of glycoside hydrolase (GH) families into clans expands the utility of
information on catalytic mechanisms of member enzymes. This issue was examined for …