High-throughput sequencing of the products of 5′ RNA ligase-mediated rapid amplification
of cDNA ends (5′ RLM-RACE) reactions (RACE-SEQ) enables the mapping and digital …

In the current ocean of high-throughput sequencing techniques, it can be overwhelming to
understand how a particular technology works, what it excels at, and what it struggles to …

Low allelic and clonal variability among endogenous RNAi targets has focused mismatch
tolerance studies to RNAi-active guide strands. However, the inherent genomic instability of …

ABSTRACT R NA-mediated oligonucleotide A nnealing, S election, and L igation (RASL-
seq) is a method to measure the expression of hundreds of genes in thousands of samples …

Large-scale RNAi screens (ie, genome-wide arrays and pools) can reveal the essential
biological functions of previously uncharacterized genes. Due to the nature of the selection …

The advent of low-cost, high-throughput sequencing technologies has elucidated targetable
cancer-specific genomic alterations and allowed the development of precision therapies and …

High throughput molecular and functional profiling of patients is a key driver of precision
medicine. DNA and RNA characterization has been enabled at unprecedented cost and …

Functional precision medicine (FPM) aims to optimize patient-specific drug selection based
on the unique characteristics of their cancer cells. Recent advancements in high throughput …

Protein-targeted small-molecule drugs may unintentionally bind intracellular RNA,
contributing to drug toxicity. Moreover, new drugs are actively sought for intentionally …

Therapeutic targeting of epigenetic modulators offers a novel approach to the treatment of
multiple diseases. The cellular consequences of chemical compounds that target epigenetic …