Background Cells are powered by a large set of macromolecular complexes, which work
together in a crowded environment. The in situ mechanisms of these complexes are unclear …

Today, cryo-electron tomography (cryo-ET) is the only technique that can provide near-
atomic resolution structural data on macromolecular complexes in situ. Owing to the strong …

Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular
structures and macromolecules in situ. Due to poor penetration of electrons through thick …

Single-particle electron cryomicroscopy is undergoing a technical revolution due to the
recent developments of direct detectors. These new recording devices detect electrons …

Cryogenic electron tomography (cryoET) is a powerful method to study the 3D structure of
biological samples in a close-to-native state. Current state-of-the-art cryoET combined with …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Highlights•Electron cryo-tomography (cryoET) reveals structures in a near-native
state.•Recent technological advances have increased the information obtainable by …

Cryo-electron tomography (cryo-ET) and subtomogram averaging (STA) are becoming the
preferred methodologies for investigating subcellular and macromolecular structures in …

Cryogenic electron tomography (cryo-ET) produces datasets tremendously rich in
information, with significant value beyond their originating study. Openly sharing such data …

Electron cryo-tomography (cryo-ET) is a technique that allows the investigation of intact
macromolecular complexes while they are in their cellular milieu. Over the years, cryo-ET …