Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

GM Baci, AA Cucu, AI Giurgiu, AS Muscă, L Bagameri… - Insects, 2021 - mdpi.com
Simple Summary One of the most powerful gene editing approaches is the CRISPR
(clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) tool …

An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites

S Ma, Y Liu, Y Liu, J Chang, T Zhang, X Wang… - Insect biochemistry and …, 2017 - Elsevier
Genome editing enabled unprecedented new opportunities for targeted genomic
engineering of a wide variety of organisms ranging from microbes, plants, animals and even …

Heritable Genome Editing with CRISPR/Cas9 in the Silkworm, Bombyx mori

W Wei, H Xin, B Roy, J Dai, Y Miao, G Gao - PloS one, 2014 - journals.plos.org
We report the establishment of an efficient and heritable gene mutagenesis method in the
silkworm Bombyx mori using modified type II clustered regularly interspaced short …

Application of the CRISPR gene‐editing technique in insect functional genome studies–a review

Y Cui, JL Sun, LL Yu - Entomologia Experimentalis et Applicata, 2017 - Wiley Online Library
The clustered regularly interspaced short palindromic repeats (CRISPR) structural family
form an acquired immune system in bacteria that is able to degrade invading virus or phage …

Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9

Y Liu, S Ma, X Wang, J Chang, J Gao, R Shi… - Insect biochemistry and …, 2014 - Elsevier
Bombyx mori is an economically important insect and a model organism for studying
lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we …

Genome editing in pests: basic science to applications

X Chen, SR Palli - Journal of Pest Science, 2024 - Springer
Recent developments in sequencing technologies produced enormous data on gene
sequences and the identity of genes in many pest insects and disease vectors. However, the …

CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns

CNT Taning, B Van Eynde, N Yu, S Ma… - Journal of insect …, 2017 - Elsevier
Discovered as a bacterial adaptive immune system, CRISPR/Cas9 (clustered, regularly
interspaced, short palindromic repeat/CRISPR associated) is being developed as an …

Progress and prospects of CRISPR/Cas systems in insects and other arthropods

D Sun, Z Guo, Y Liu, Y Zhang - Frontiers in physiology, 2017 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-
associated gene Cas9 represent an invaluable system for the precise editing of genes in …

CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

JJ Li, Y Shi, JN Wu, H Li, G Smagghe, TX Liu - Journal of insect physiology, 2021 - Elsevier
Clustered regularly spaced short palindrome repeats (CRISPR) structure family forms the
acquired immune system in bacteria and archaea. Recent advances in CRISPR/Cas …

CRISPR/Cas9 Genome Editing Introduction and Optimization in the Non-model Insect Pyrrhocoris apterus

J Kotwica-Rolinska, L Chodakova, D Chvalova… - Frontiers in …, 2019 - frontiersin.org
The CRISPR/Cas9 technique is widely used in experimentation with human cell lines as
well as with other model systems, such as mice Mus musculus, zebrafish Danio reiro, and …