Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis

T Shirai, K Fujimura, C Furusawa, K Nagahisa… - Microbial cell …, 2007 - Springer
Background Corynebacterium glutamicum has several anaplerotic pathways (anaplerosis),
which are essential for the productions of amino acids, such as lysine and glutamate. It is still …

Importance of phosphoenolpyruvate carboxylase of Corynebacterium glutamicum during the temperature triggered glutamic acid fermentation

S Delaunay, D Uy, MF Baucher, JM Engasser… - Metabolic …, 1999 - Elsevier
To give clues about the respective importance of phosphoenolpyruvate carboxylase (PEPc)
and pyruvate carboxylase (Pc) in Corynebacterium glutamicum metabolism during a …

Changes in enzyme activities at the pyruvate node in glutamate-overproducing Corynebacterium glutamicum

T Hasegawa, KI Hashimoto, H Kawasaki… - Journal of bioscience …, 2008 - Elsevier
Glutamate is industrially produced by fermentation using Corynebacterium glutamicum. The
key factor for efficient glutamate production by this microorganism has been considered to …

Molecular Mechanisms and Metabolic Engineering of Glutamate Overproduction in Corynebacterium glutamicum

T Hirasawa, J Kim, T Shirai, C Furusawa… - Reprogramming …, 2012 - Springer
Glutamate is a commercially important chemical. It is used as a flavor enhancer and is a
major raw material for producing industrially useful chemicals. A coryneform bacterium …

Pathway analysis and metabolic engineering in Corynebacterium glutamicum

H Sahm, L Eggeling, AA Graaf - 2000 - degruyter.com
Abstract The Gram-positive bacterium Corynebacterium glutamicum is used for the industrial
production of amino acids, eg of L-glutamate and L-lysine. During the last 15 years, genetic …

Double deletion of dtsR1 and pyc induce efficient l-glutamate overproduction in Corynebacterium glutamicum

W Yao, X Deng, H Zhong, M Liu, P Zheng… - Journal of Industrial …, 2009 - academic.oup.com
Corynebacterium glutamicum strains are used for the fermentative production of l-glutamate.
Five C. glutamicum deletion mutants were isolated by two rounds of selection for …

Gene expression of Corynebacterium glutamicum in response to the conditions inducing glutamate overproduction

M Kataoka, KI Hashimoto, M Yoshida… - Letters in applied …, 2006 - academic.oup.com
Aim: The ultimate aim is to elucidate the molecular mechanisms for glutamate
overproduction by Corynebacterium glutamicum. Methods and Results: Gene expression in …

Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum

H Sato, K Orishimo, T Shirai, T Hirasawa… - Journal of bioscience …, 2008 - Elsevier
Corynebacterium glutamicum is a biotin auxotrophic bacterium in which glutamate
production is induced under biotin-limited conditions. During glutamate production …

Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum

S Delaunay, P Daran-Lapujade… - Journal of Industrial …, 2004 - academic.oup.com
The glutamate-producing bacterium, Corynebacterium glutamicum is known to possess two
anaplerotic enzymes: pyruvate carboxylase (Pc) and phosphoenolpyruvate carboxylase …

Response of the central metabolism of Corynebacterium glutamicum to different flux burdens

A Marx, K Striegel, AA de Graaf… - Biotechnology and …, 1997 - Wiley Online Library
To evaluate the importance of reactions within the central metabolism under different flux
burdens the fluxes within the pentose phosphate pathway (PPP), as well as the other …