A novel multiplex qRT-PCR assay to detect SARS-CoV-2 infection: high sensitivity and increased testing capacity

S Petrillo, G Carrà, P Bottino, E Zanotto, MC De Santis… - Microorganisms, 2020 - mdpi.com
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is essential to limit the spread of the global pandemic we are facing. Quantitative real …

Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples

M Laverack, RL Tallmadge, R Venugopalan, B Cronk… - Archives of …, 2021 - Springer
The aim of this study was to identify and validate a sensitive, high-throughput, and cost-
effective SARS-CoV-2 real-time RT-PCR assay to be used as a surveillance and diagnostic …

High sensitivity-low cost detection of SARS-CoV-2 by two steps end point RT-PCR with agarose gel electrophoresis visualization

S Figueroa, B Freire-Paspuel, P Vega-Mariño… - Scientific Reports, 2021 - nature.com
More than one year since Coronavirus disease 2019 (COVID-19) pandemic outbreak, the
gold standard technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …

Clinical usefulness of extraction-free PCR assay to detect SARS-CoV-2

YK Kim, SH Chang - Journal of Virological Methods, 2021 - Elsevier
Due to the coronavirus disease 2019 pandemic, the demand for an easily accessible high-
throughput screening test is increasing. We aimed to evaluate the usefulness of the extrac …

Diagnostic power of one-step and two-step RT-qPCR methods to SARS‑CoV‑2 detection

A Malekshahi, S Khanizadeh, S Fallahi, G Talei… - BMC infectious …, 2022 - Springer
Abstract Background Coronavirus-2019 (COVID-2019) is a novel coronavirus known as
Acute Respiratory Syndrome (SARS-CoV-2). The premier standard test for SARS-CoV-2 …

Laboratory verification of an RT‐PCR assay for SARS‐CoV‐2

Y Wu, W Xu, Z Zhu, X Xia - Journal of Clinical Laboratory …, 2020 - Wiley Online Library
Background Reverse transcription‐polymerase chain reaction (RT‐PCR) is an extremely
common clinical method for detecting pathogens, particularly for emerging infectious …

[HTML][HTML] SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay

MD Buck, EZ Poirier, A Cardoso… - Wellcome Open …, 2021 - ncbi.nlm.nih.gov
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to
accurately identify infected individuals. The vast majority of patient samples is assessed for …

Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

U Eberle, C Wimmer, I Huber, A Neubauer-Juric… - European Journal of …, 2021 - Springer
To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the
detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating …

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples

VL Dao Thi, K Herbst, K Boerner, M Meurer… - Science translational …, 2020 - science.org
The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV-2 (severe
acute respiratory syndrome coronavirus 2) coronavirus is a major public health challenge …

Optimized qRT-PCR approach for the detection of intra-and extra-cellular SARS-CoV-2 RNAs

T Toptan, S Hoehl, S Westhaus, D Bojkova… - International journal of …, 2020 - mdpi.com
The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease
COVID-19, which has become a global concern due to its rapid spread. Meanwhile …