Daidzein reductase of Eggerthella sp. YY7918, its octameric subunit structure containing FMN/FAD/4Fe-4S, and its enantioselective production of R …

Y Kawada, T Goshima, R Sawamura… - Journal of bioscience …, 2018 - Elsevier
S-Equol is a metabolite of daidzein, a type of soy isoflavone, and three reductases are
involved in the conversion of daidzein by specific intestinal bacteria. S-Equol is thought to …

Characterization and Identification of a New Daidzein Reductase Involved in (S)-Equol Biosynthesis in Clostridium sp. ZJ6

Y Hu, C Yang, C Song, W Zhong, B Li, L Cao… - Frontiers in …, 2022 - frontiersin.org
(S)-equol (EQ) is an isoflavone with high estrogen-like activity in the human body, and is
only produced by some gut bacteria in vivo. It plays an important role in maintaining …

P212A Mutant of Dihydrodaidzein Reductase Enhances (S)-Equol Production and Enantioselectivity in a Recombinant Escherichia coli Whole-Cell Reaction System

PG Lee, J Kim, EJ Kim, EO Jung… - Applied and …, 2016 - Am Soc Microbiol
ABSTRACT (S)-Equol, a gut bacterial isoflavone derivative, has drawn great attention
because of its potent use for relieving female postmenopausal symptoms and preventing …

Cloning and expression of a novel NADP (H)-dependent daidzein reductase, an enzyme involved in the metabolism of daidzein, from equol-producing Lactococcus …

Y Shimada, S Yasuda, M Takahashi… - Applied and …, 2010 - Am Soc Microbiol
Equol is a metabolite produced from daidzein by enteric microflora, and it has attracted a
great deal of attention because of its protective or ameliorative ability against several sex …

The production of S-equol from daidzein is associated with a cluster of three genes in Eggerthella sp. YY7918

Y Kawada, S Yokoyama, E Yanase, T Niwa… - … of microbiota, food …, 2016 - jstage.jst.go.jp
Daidzein (DZN) is converted to equol (EQL) by intestinal bacteria. We previously reported
that Eggerthella sp. YY7918, which is found in human feces, is an EQL-producing bacterium …

Identification of a novel dihydrodaidzein racemase essential for biosynthesis of equol from daidzein in Lactococcus sp. strain 20-92

Y Shimada, M Takahashi, N Miyazawa… - Applied and …, 2012 - Am Soc Microbiol
Equol is metabolized from daidzein, a soy isoflavone, by the gut microflora. In this study, we
identified a novel dihydrodaidzein racemase (l-DDRC) that is involved in equol biosynthesis …

Exploring functional genes' correlation with (S)-equol concentration and new daidzein racemase identification

YF Hu, S Luo, SQ Wang, KX Chen… - Applied and …, 2024 - Am Soc Microbiol
With its estrogenic activity,(S)-equol plays an important role in maintaining host health and
preventing estrogen-related diseases. Exclusive production occurs through the …

Isoflavone daidzein: chemistry and bacterial metabolism

MH Kim, JH Han, SU Kim - Journal of applied biological chemistry, 2008 - dbpia.co.kr
Isoflavone daidzein is a phytoestrogen widely distributed in Leguminosae and is especially
rich in the soybean. The C6-C3 (rings B and C) unit of isoflavones is derived from the …

Biosynthesis of (−)-5-hydroxy-equol and 5-hydroxy-dehydroequol from soy isoflavone, genistein using microbial whole cell bioconversion

PG Lee, J Kim, EJ Kim, SH Lee, KY Choi… - ACS chemical …, 2017 - ACS Publications
Equols are isoflavandiols formed by reduction of soy isoflavones such as daidzein and
genistein by gut microorganisms. These phytoestrogens are of interest for their various …

Reduction of soy isoflavones by use of Escherichia coli whole‐cell biocatalyst expressing isoflavone reductase under aerobic conditions

YN Gao, QH Hao, HL Zhang, B Zhou… - Letters in Applied …, 2016 - academic.oup.com
Soy isoflavone metabolites are currently receiving much attention due to the stronger and
wider bioactivities than that of isoflavones. Therefore, biosynthesis of isoflavone metabolites …