Colorimetric RT-LAMP and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples

K Herbst, M Meurer, D Kirrmaier, S Anders, M Knop… - Bio …, 2021 - bio-protocol.org
During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to
rapidly test large numbers of people are needed. As a faster and less resource-demanding …

Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays …

E Gonzalez-Gonzalez, IM Lara-Mayorga… - Analytical …, 2021 - pubs.rsc.org
We demonstrate a loop-mediated isothermal amplification (LAMP) method to detect and
amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that …

A novel multiplex qRT-PCR assay to detect SARS-CoV-2 infection: high sensitivity and increased testing capacity

S Petrillo, G Carrà, P Bottino, E Zanotto, MC De Santis… - Microorganisms, 2020 - mdpi.com
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is essential to limit the spread of the global pandemic we are facing. Quantitative real …

Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA

Y Zhang, NA Tanner - Biotechniques, 2021 - Taylor & Francis
The ongoing pandemic has demonstrated the utility of widespread surveillance and
diagnostic detection of the novel SARS-CoV-2. Reverse-transcription loop-mediated …

[HTML][HTML] Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

JJ LeBlanc, JB Gubbay, Y Li, R Needle… - Journal of Clinical …, 2020 - Elsevier
With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential.
This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV …

Loop mediated isothermal amplification (LAMP) assays as a rapid diagnostic for COVID-19

J Kashir, A Yaqinuddin - Medical hypotheses, 2020 - Elsevier
Recently, a novel coronavirus (SARS-CoV-2; coronavirus disease 2019, COVID-19) has
emerged, rapidly spreading and severely straining the capacity of the global health …

CLEVER assay: A visual and rapid RNA extraction‐free detection of SARS‐CoV‐2 based on CRISPR‐Cas integrated RT‐LAMP technology

A Bhatt, Z Fatima, M Ruwali, CS Misra… - Journal of applied …, 2022 - academic.oup.com
Aim The current scenario of COVID‐19 pandemic has presented an almost insurmountable
challenge even for the most sophisticated hospitals equipped with modern biomedical …

[HTML][HTML] A comparative study of real-time RT-PCR–based SARS-CoV-2 detection methods and its application to human-derived and surface swabbed material

A Tastanova, CI Stoffel, A Dzung, PF Cheng… - The Journal of Molecular …, 2021 - Elsevier
Real-time RT-PCR remains a gold standard in the detection of various viral diseases. In the
coronavirus 2019 pandemic, multiple RT-PCR–based tests were developed to screen for …

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious …

MM Islam, D Koirala - Analytica chimica acta, 2022 - Elsevier
As the COVID-19 pandemic continues to affect human health across the globe rapid, simple,
point-of-care (POC) diagnosis of infectious viruses such as SARS-CoV-2 remains …

Detecting SARS-CoV-2 at point of care: preliminary data comparing loop-mediated isothermal amplification (LAMP) to PCR

MF Österdahl, KA Lee, MN Lochlainn, S Wilson… - MedRxiv, 2020 - medrxiv.org
Background The need for a fast and reliable test for COVID-19 is paramount in managing
the current pandemic. A cost effective and efficient diagnostic tool as near to the point of care …