PURPOSE AND APPROPRIATE SAMPLE TYPES This 40‐color flow cytometry‐based panel
was developed for in‐depth immunophenotyping of the major cell subsets present in human …

High‐dimensional mass cytometry data potentially enable a comprehensive characterization
of immune cells. In order to positively affect clinical trials and translational clinical research …

Standardization of immunophenotyping requires careful attention to reagents, sample
handling, instrument setup, and data analysis, and is essential for successful cross-study …

The development of flow cytometric biomarkers in human studies and clinical trials has been
slowed by inconsistent sample processing, use of cell surface markers, and reporting of …

High‐dimensional single‐cell data has become an important tool in unraveling the
complexity of the immune system and its involvement in homeostasis and a large array of …

Although many flow cytometers can analyze 30–50 parameters, it is still challenging to
develop a 40+ color panel for the phenotyping of immune cells using fluorochrome …

Introduction Phagocytes such as granulocytes and monocytes are fundamental players in
the innate immune system. Activation of these cells can be quantified by the measurement of …

Background Cytokine flow cytometry (CFC) or intracellular cytokine staining (ICS) can
quantitate antigen-specific T cell responses in settings such as experimental vaccination …

Abstract The EU-supported EuroFlow Consortium aimed at innovation and standardization
of immunophenotyping for diagnosis and classification of hematological malignancies by …

Monitoring changes in human immune cell populations such as lymphocytes, monocytes,
and dendritic cells (DCs) during infectious diseases like human immunodeficiency virus …