Protein conjugations at post-translational levels are known to be essential to protein stability
and function. Recently, it has been proven that the split protein CnaB2 (SpyTag/SpyCatcher …

Genetically encoded peptide‐protein coupling reactions, such as the SpyTag/SpyCatcher
chemistry, are recent additions to the expanding toolbox of protein bioconjugation. The …

Many recombinant proteins have been successfully produced in silkworm larvae or pupae
and used for academic and industrial purposes. Several recombinant proteins produced by …

A ligation independent cloning (LIC) system has been developed to facilitate the rapid and
high-efficiency cloning of genes in a Bombyx mori expression system. This system was …

Fundamental and applied studies of silkworms have entered the functional genomics era.
Here, we report a multi-gene expression system (MGES) based on 2A self-cleaving peptide …

Objective To examine the feasibility of chitosan as an alternative transfection reagent
candidate for protein expression in Bm5 cells and silkworm larvae using recombinant …

Site-specific cross-linking techniques between proteins and additional functional groups
have become increasingly important for expanding the utility of proteins in biochemistry and …

The domesticated silkworm, Bombyx mori, is an economically important insect that
synthesizes large amounts of silk proteins in its silk gland to make cocoons. In recent years …

Based on the specific and spontaneous formation of isopeptide bonds by
SpyCatcher/SpyTag, we have developed a one-step method for purification and …

The piggyBac transposable element was successfully used for generation of the transgenic
silkworm, Bombyx mori. The EGFP was adopted in this study as a screening marker in …