[PDF][PDF] Study on the efficient purification of recombinant proteins from the silkworm expression system

R Minkner - 2019 - shizuoka.repo.nii.ac.jp
3.2. 1. GFPuv-β1, 3-N-acetylglucosaminyltransferase 2 (GFP-β3GnT2) Haemolymph
containing infective recombinant baculovirus for GFPuv-β1, 3-N …

Progress and application prospect of transgenic silkworm.

ZQS Zhou QiSheng, YQ Yu Qi, LQX Liu QingXin - 2011 - cabidigitallibrary.org
Transgenic silkworm, Bombyx mori was generated by transferring foreign genes to its
chromosome with molecular biological techniques and creating new traits and products …

Construction of transgenic silkworm gene targeting vectors based on fibroin light chain

Z Chenggang, J Yongfeng, S Feng… - Nong ye Sheng wu ji …, 2002 - europepmc.org
The promoter of silkworm (Bombyx mori) fibroin is one of the most strong promoters in
nature. It is important that the heterologous gene expressed in the silk gland under the …

Silkworm expression system as a platform technology in life science

T Kato, M Kajikawa, K Maenaka, EY Park - Applied microbiology and …, 2010 - Springer
Many recombinant proteins have been successfully produced in silkworm larvae or pupae
and used for academic and industrial purposes. Several recombinant proteins produced by …

Advances in research of silk gland bioreactor of piggyBac-mediated transgenic silkworm.

ZBX Zhong BoXiong, WH Wei Hao… - 2011 - cabidigitallibrary.org
This paper reviewed three key steps of recombinant protein expression by silk gland
bioreactor of piggy Bac-mediated transgenic silkworm. Firstly, the efficient insertion of …

Research on the production of useful protein using silkworm (Bombyx mori) as a bioreactor

Y Ruili, J Yongfeng, W Yucheng, Z Yaozhou… - Journal of Zhejiang …, 2001 - europepmc.org
Summarized the basic principle, production steps, achievements of expression foreign
genes and pharmaceuticals development using Silkworm/BmNPV Expression System, and …

piggyBac 转座子介导的转基因家蚕丝腺生物反应器研究进展

钟伯雄, 危浩, 庄兰芳 - 中国农业科学, 2010 - chinaagrisci.com
This paper reviewed three key steps of recombinant protein expression by silk gland
bioreactor of piggyBac-mediated transgenic silkworm. Firstly, the efficient insertion of foreign …

Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system

Y Miao, Y Zhang, K Nakagaki, T Zhao, A Zhao… - Applied microbiology …, 2006 - Springer
Bombyx mori nuclear polyhedrosis virus (BmNPV) baculovirus expression system (BES) has
a lot of advantages such as high expression efficiency, convenience, and low feeding cost …

Comparison of the efficiencies of different affinity tags in the purification of a recombinant secretory protein expressed in silkworm larval hemolymph

T Dojima, T Nishina, T Kato, H Ueda… - Biotechnology and …, 2009 - Springer
Silkworms are useful bioreactors for heterologous protein expression when used in
conjunction with the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system. However …

Establishment of the BacMam system using silkworm baculovirus

A Imai, T Tadokoro, S Kita, M Horiuchi… - Biochemical and …, 2016 - Elsevier
The BacMam system uses modified insect viruses (baculoviruses) as vehicles to efficiently
deliver genes for expression in mammalian cells. The technique can be widely applied to …