The Caulobacter crescentus holdfast: Identification of holdfast attachment complex genes

HD Kurtz Jr, J Smith - FEMS microbiology letters, 1994 - academic.oup.com
Caulobacters are biofilm bacteria that attach to surfaces via a holdfast, an adhesive
expressed at discrete cell surface sites. We have described a cluster of at least three genes …

A localized multimeric anchor attaches the Caulobacter holdfast to the cell pole

GG Hardy, RC Allen, E Toh, M Long… - Molecular …, 2010 - Wiley Online Library
Caulobacter crescentus attachment is mediated by the holdfast, a complex of
polysaccharide anchored to the cell by HfaA, HfaB and HfaD. We show that all three proteins …

HfaE is a component of the holdfast anchor complex that tethers the holdfast adhesin to the cell envelope

NK Chepkwony, GG Hardy, YV Brun - Journal of Bacteriology, 2022 - Am Soc Microbiol
Bacteria use adhesins to colonize different surfaces and form biofilms. The species of the
Caulobacterales order use a polar adhesin called holdfast, composed of polysaccharides …

A cryptic transcription factor regulates Caulobacter adhesin development

M McLaughlin, DM Hershey, LM Reyes Ruiz… - PLoS …, 2022 - journals.plos.org
Alphaproteobacteria commonly produce an adhesin that is anchored to the exterior of the
envelope at one cell pole. In Caulobacter crescentus this adhesin, known as the holdfast …

A multiprotein complex anchors adhesive holdfast at the outer membrane of Caulobacter crescentus

NI Sulkowski, GG Hardy, YV Brun… - Journal of …, 2019 - Am Soc Microbiol
Adhesion allows microbes to colonize surfaces and is the first stage in biofilm formation.
Stable attachment of the freshwater alphaproteobacterium Caulobacter crescentus to …

The HfaB and HfaD adhesion proteins of Caulobacter crescentus are localized in the stalk

JL Cole, GG Hardy, D Bodenmiller, E Toh… - Molecular …, 2003 - Wiley Online Library
The differentiating bacterium Caulobacter crescentus produces two different cell types at
each cell division, a motile swarmer cell and an adhesive stalked cell. The stalked cell …

Mutations in sugar-nucleotide synthesis genes restore holdfast polysaccharide anchoring to Caulobacter crescentus holdfast anchor mutants

GG Hardy, E Toh, C Berne, YV Brun - Journal of bacteriology, 2018 - Am Soc Microbiol
Attachment is essential for microorganisms to establish interactions with both biotic and
abiotic surfaces. Stable attachment of Caulobacter crescentus to surfaces requires an …

Analysis of a Caulobacter crescentus gene cluster involved in attachment of the holdfast to the cell

HD Kurtz Jr, J Smith - Journal of bacteriology, 1992 - Am Soc Microbiol
Caulobacter crescentus firmly adheres to surfaces with a structure known as the holdfast,
which is located at the flagellar pole of swarmer cells and at the stalk tip in stalked cells. A …

A Genome-Wide Analysis of Adhesion in Caulobacter crescentus Identifies New Regulatory and Biosynthetic Components for Holdfast Assembly

DM Hershey, A Fiebig, S Crosson - MBio, 2019 - Am Soc Microbiol
Due to their intimate physical interactions with the environment, surface polysaccharides are
critical determinants of fitness for bacteria. Caulobacter crescentus produces a specialized …

Identification of Genes Required for Synthesis of the Adhesive Holdfast in Caulobacter crescentus

CS Smith, A Hinz, D Bodenmiller, DE Larson, YV Brun - 2003 - Am Soc Microbiol
Adhesion to both abiotic and biotic surfaces by the gram-negative prothescate bacterium
Caulobacter crescentus is mediated by a polar organelle called the “holdfast,” which …